Display options
Cite
Fujihashi K, Taguchi T, Aicher WK, et al. Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help. J Exp Med. 1992;175(3):695-707doi: 10.1084/jem.175.3.695.
Fujihashi, K., Taguchi, T., Aicher, W. K., McGhee, J. R., Bluestone, J. A., Eldridge, J. H., & Kiyono, H. (1992). Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help. The Journal of experimental medicine, 175(3), 695-707. https://doi.org/10.1084/jem.175.3.695
Fujihashi, K, et al. "Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help." The Journal of experimental medicine vol. 175,3 (1992): 695-707. doi: https://doi.org/10.1084/jem.175.3.695
Fujihashi K, Taguchi T, Aicher WK, McGhee JR, Bluestone JA, Eldridge JH, Kiyono H. Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help. J Exp Med. 1992 Mar 01;175(3):695-707. doi: 10.1084/jem.175.3.695. PMID: 1531495; PMCID: PMC2119151.
Copy Download .nbib Format: NLM
Share it on
Full text links
Silverchair Information Systems Free PMC Article

J Exp Med. 1992 Mar 01;175(3):695-707. doi: 10.1084/jem.175.3.695.

Immunoregulatory functions for murine intraepithelial lymphocytes: gamma/delta T cell receptor-positive (TCR+) T cells abrogate oral tolerance, while alpha/beta TCR+ T cells provide B cell help.

The Journal of experimental medicine

K Fujihashi, T Taguchi, W K Aicher, J R McGhee, J A Bluestone, J H Eldridge, H Kiyono

Affiliations

  1. Department of Oral Biology, University of Alabama, Birmingham 35294.

PMID: 1531495 PMCID: PMC2119151 DOI: 10.1084/jem.175.3.695
Free PMC Article

Abstract

Past work has shown that a subset of effector T cells with unique characteristics could abrogate hapten- or antigen-induced tolerance, and the reconstitution of this immune response has been termed contrasuppression. We have studied contrasuppression in a model of oral tolerance (OT) in which adoptively transferred antigen-specific T contrasuppressor (Tcs) cells reverse OT and result in antibody responses to the eliciting antigen. In the present study, we show that murine intraepithelial lymphocytes (IELs) from mice orally immunized with sheep red blood cells (SRBC) contain T cells that exhibit Tcs cell activity. This effect was mediated by CD3+ gamma/delta T cell receptor-positive (TCR+), but not alpha/beta TCR+ T cells, and gamma/delta TCR+ Tcs cells were associated with both the CD4-,CD8+ and CD4-,CD8- (double-negative) IEL fractions. The CD4-,CD8+ gamma/delta TCR+ IELs were further separated into Vicia villosa-adherent and -nonadherent fractions. Adoptive transfer of V. villosa-adherent gamma/delta TCR+ T cells to mice with OT to SRBC resulted in splenic IgA, IgM, and IgG subclass anti-SRBC responses, while V. villosa-nonadherent gamma/delta TCR+ T cells were without activity. The gamma/delta TCR+ IELs did not support in vitro antibody responses in B cell cultures, while alpha/beta TCR+ IELs were effective T helper cells. Further, cytokine production by the gamma/delta TCR+ IELs was examined, and the gamma/delta TCR+ V. villosa-adherent fraction, which possessed contrasuppressor function, contained low levels of IL-5 mRNA and small numbers of IL-5-producing cells when compared with alpha/beta TCR+ IELs and V. villosa-nonadherent gamma/delta TCR+ IELs. Our results now show that mouse IELs contain two distinct types of T cells that function in the immune response, e.g., alpha/beta TCR+ T cells that produce IL-5 and function as helper cells, and gamma/delta TCR+ T cells that restore antibody responses in mice that had been orally tolerized with antigen.

Similar articles

Show all 31 similar articles

References

  1. Immunol Rev. 1979;47:63-90 - PubMed
  2. J Exp Med. 1986 Aug 1;164(2):501-16 - PubMed
  3. Eur J Immunol. 1990 Mar;20(3):703-6 - PubMed
  4. J Exp Med. 1991 Feb 1;173(2):471-81 - PubMed
  5. J Immunol. 1990 Oct 1;145(7):2010-9 - PubMed
  6. Int Immunol. 1990;2(4):361-5 - PubMed
  7. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):43-7 - PubMed
  8. J Exp Med. 1990 Apr 1;171(4):1015-26 - PubMed
  9. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8 - PubMed
  10. J Exp Med. 1987 Nov 1;166(5):1229-44 - PubMed
  11. Adv Exp Med Biol. 1987;216A:465-73 - PubMed
  12. J Immunol. 1988 Mar 1;140(5):1385-92 - PubMed
  13. Nature. 1988 Jun 30;333(6176):855-8 - PubMed
  14. J Immunol. 1988 Sep 1;141(5):1576-81 - PubMed
  15. J Immunol. 1988 Sep 15;141(6):2035-42 - PubMed
  16. Nature. 1986 Apr 3-9;320(6061):451-4 - PubMed
  17. J Immunol. 1987 Nov 15;139(10):3251-9 - PubMed
  18. Eur J Immunol. 1985 Mar;15(3):216-21 - PubMed
  19. Clin Exp Immunol. 1972 Dec;12(4):477-88 - PubMed
  20. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
  21. J Exp Med. 1984 Dec 1;160(6):1931-6 - PubMed
  22. J Immunol. 1983 Nov;131(5):2445-51 - PubMed
  23. J Exp Med. 1984 May 1;159(5):1560-5 - PubMed
  24. Immunology. 1981 Oct;44(2):367-71 - PubMed
  25. Hybridoma. 1982;1(2):125-31 - PubMed
  26. J Biol Chem. 1982 Aug 10;257(15):8569-72 - PubMed
  27. J Exp Med. 1980 Dec 1;152(6):1459-72 - PubMed
  28. J Immunol. 1990 Jul 1;145(1):20-7 - PubMed
  29. Cell. 1990 Oct 19;63(2):333-40 - PubMed
  30. J Immunol. 1990 Sep 1;145(5):1369-75 - PubMed
  31. Nature. 1988 Dec 1;336(6198):479-81 - PubMed
  32. J Immunol. 1989 Apr 15;142(8):2736-42 - PubMed
  33. J Exp Med. 1989 May 1;169(5):1847-51 - PubMed
  34. Nature. 1989 Oct 5;341(6241):447-50 - PubMed
  35. Annu Rev Immunol. 1989;7:145-73 - PubMed
  36. J Immunol. 1989 Dec 1;143(11):3415-22 - PubMed
  37. Curr Top Microbiol Immunol. 1989;146:13-25 - PubMed
  38. J Exp Med. 1989 Apr 1;169(4):1491-6 - PubMed
  39. J Exp Med. 1989 Jun 1;169(6):2133-48 - PubMed
  40. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7388-92 - PubMed
  41. J Exp Med. 1986 Jul 1;164(1):309-14 - PubMed
  42. J Immunol. 1986 Mar 15;136(6):2121-6 - PubMed
  43. J Immunol. 1991 Dec 1;147(11):3736-44 - PubMed

Substances

MeSH terms

Publication Types

Grant support

LinkOut - more resources