Nihon Sanka Fujinka Gakkai Zasshi. 1991 May;43(5):563-6.
Nihon Sanka Fujinka Gakkai zasshi
H Kanoh
PMID: 1647431
Primary culture of human uterine endocervical epithelial cells was performed. When cells were cultured on a plastic dish, these cells developed into a flat form, then lost their proliferative ability in a short time during continuous subculture. On the other hand, when cells were cultured on collagen gel and subcultured by explanation of this cell containing gel, the cells took on a roughly ovoid rather than a flat shape and active proliferation was attained which maintained confluency for as long as 8 weeks. Furthermore by adopting the Simian Virus 40 in the culture system for these cells, it was possible to extend the lifespan to approximately twice that of the control cell group.
