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Kessler TL, Markus G. Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells. Semin Thromb Hemost. 1991;17(3):217-24doi: 10.1055/s-2007-1002612.
Kessler, T. L., & Markus, G. (1991). Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells. Seminars in thrombosis and hemostasis, 17(3), 217-24. https://doi.org/10.1055/s-2007-1002612
Kessler, T L, and Markus, G. "Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells." Seminars in thrombosis and hemostasis vol. 17,3 (1991): 217-24. doi: https://doi.org/10.1055/s-2007-1002612
Kessler TL, Markus G. Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells. Semin Thromb Hemost. 1991 Jul;17(3):217-24. doi: 10.1055/s-2007-1002612. PMID: 1796295.
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Georg Thieme Verlag Stuttgart, New York

Semin Thromb Hemost. 1991 Jul;17(3):217-24. doi: 10.1055/s-2007-1002612.

Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells.

Seminars in thrombosis and hemostasis

T L Kessler, G Markus

Affiliations

  1. Department of Experimental Biology, Roswell Park Cancer Institute, New York State Department of Health, Buffalo 14263.

PMID: 1796295 DOI: 10.1055/s-2007-1002612

Abstract

The signal transduction pathways of urokinase (u-PA) in A431 cells, a human epidermoid carcinoma cell line, were studied using the inducers EGF and TPA. Based on the following observations, we conclude that the two pathways are different: (1) The effects are additive; (2) EGF induction of u-PA was not compromised either by down-regulation of PKC or selective inhibition of the same by H7; (3) 8-Br-cAMP has no effect by itself; however, it inhibits the EGF effect and doubles the TPA induction of u-PA; (4) after EGF-induced tyrosine phosphorylations are completed, addition of TPA can still induce u-PA, an effect that can be blocked by the addition of the tyrosine phosphorylation inhibitor, genistein, indicating that the two agents induce different tyrosine phosphorylation; (5) in 2-dimensional electrophoresis of 32P-labeled cell lysates, inducer-specific differences in the intensity of spots can be observed. Some of the spots weakened by genistein (tyrosine phosphorylations) are different, depending on the inducer used.

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