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de Roos A, Mohanlal RW, van Vaals JJ, et al. Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion. Invest Radiol. 1991;26(12):1060-4doi: 10.1097/00004424-199112000-00005.
de Roos, A., Mohanlal, R. W., van Vaals, J. J., Bergman, A. H., Doornbos, J., Matheijssen, N. A., van der Wall, E. E., & van der Laarse, A. (1991). Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion. Investigative radiology, 26(12), 1060-4. https://doi.org/10.1097/00004424-199112000-00005
de Roos, A, et al. "Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion." Investigative radiology vol. 26,12 (1991): 1060-4. doi: https://doi.org/10.1097/00004424-199112000-00005
de Roos A, Mohanlal RW, van Vaals JJ, Bergman AH, Doornbos J, Matheijssen NA, van der Wall EE, van der Laarse A. Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion. Invest Radiol. 1991 Dec;26(12):1060-4. doi: 10.1097/00004424-199112000-00005. PMID: 1765438.
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Invest Radiol. 1991 Dec;26(12):1060-4. doi: 10.1097/00004424-199112000-00005.

Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion.

Investigative radiology

A de Roos, R W Mohanlal, J J van Vaals, A H Bergman, J Doornbos, N A Matheijssen, E E van der Wall, A van der Laarse

Affiliations

  1. Department of Diagnostic Radiology, University Hospital Leiden, The Netherlands.

PMID: 1765438 DOI: 10.1097/00004424-199112000-00005

Abstract

The objective of this study was to assess the potential of gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) to identify myocardial ischemia and reperfusion in the isolated rat heart model. Ischemia was induced by reducing the perfusion pressure from 80 to 30 mm Hg for 2 hours. Hearts were not reperfused, or were reperfused for 20 minutes or for 2 hours. Perfusion was performed with Evans blue dye and/or Gd-DTPA for 3 minutes. Twenty isolated rat hearts were perfused according to the Langendorff method, and divided into five groups according to the perfusion status and the use of Gd-DTPA and/or Evans blue as perfusion markers. The Evans blue distribution in the hearts was assessed by point-counting volumetry. The Gd-DTPA distribution was assessed by magnetic resonance microimaging at 6.3 T field strength. Evans blue staining clearly identified areas with "no flow" or "no reflow." Perfusion with Gd-DTPA enhanced signal intensity significantly, both in ischemic and reperfused myocardium. Signal intensity in hearts reperfused for 2 hours was increased significantly compared to nonreperfused ischemic hearts, but not to ischemic hearts reperfused for 20 minutes. Magnetic resonance imaging with the aid of Gd-DTPA can identify ischemia and reperfusion in the isolated rat heart, dependent on residual perfusion.

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