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Simpson RJ, Cooper CE, Raja KB, et al. Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice. Biochim Biophys Acta. 1992;1156(1):19-26doi: 10.1016/0304-4165(92)90090-h.
Simpson, R. J., Cooper, C. E., Raja, K. B., Halliwell, B., Evans, P. J., Aruoma, O. I., Singh, S., & Konijn, A. M. (1992). Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice. Biochimica et biophysica acta, 1156(1), 19-26. https://doi.org/10.1016/0304-4165(92)90090-h
Simpson, R J, et al. "Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice." Biochimica et biophysica acta vol. 1156,1 (1992): 19-26. doi: https://doi.org/10.1016/0304-4165(92)90090-h
Simpson RJ, Cooper CE, Raja KB, Halliwell B, Evans PJ, Aruoma OI, Singh S, Konijn AM. Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice. Biochim Biophys Acta. 1992 Dec 08;1156(1):19-26. doi: 10.1016/0304-4165(92)90090-h. PMID: 1335284.
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Elsevier Science

Biochim Biophys Acta. 1992 Dec 08;1156(1):19-26. doi: 10.1016/0304-4165(92)90090-h.

Non-transferrin-bound iron species in the serum of hypotransferrinaemic mice.

Biochimica et biophysica acta

R J Simpson, C E Cooper, K B Raja, B Halliwell, P J Evans, O I Aruoma, S Singh, A M Konijn

Affiliations

  1. Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, UK.

PMID: 1335284 DOI: 10.1016/0304-4165(92)90090-h

Abstract

Serum from homozygous hypotransferrinaemic mice (a mixed group of males and females, aged 6-8 wk) was found to contain low levels of iron (mean 0.9 +/- 0.5 microM (SEM, n = 4), as assayed by conventional serum iron assays. Similarly, low levels of non-transferrin-bound iron were determined with a nitrilotriacetate chelation assay (1.3 +/- 0.4 microM, n = 4) (Singh, S., Hider, R.C. and Porter, J.B. (1990) Analytical Biochemistry 186, 320-323). Mononuclear Fe (citrate) was undectable by electron paramagnetic resonance spectroscopy (EPR). Significantly larger quantities of iron (16 +/- 5 microM, n = 8) were detected by the bleomycin assay (Gutteridge, J.M.C., Rowley, D.A. and Halliwell, B. (1981) Biochemical Journal 199, 263-265), while non-haem iron assay or atomic absorption spectrophotometry revealed up to 96 microM iron. Haemoglobin iron was detectable at approximately 10 microM by spectrophotometry. Ferri-haem was undetectable by EPR spectroscopy. Serum ferritin levels of 641 +/- 128 micrograms/l (n = 14) in hypotransferrinaemic mice (wild-types 44 +/- 6 micrograms/l, n = 14) were observed and these cannot account for the non-transferrin-bound iron. Hypotransferrinaemic mouse serum therefore contains large quantities of non-transferrin-bound iron which is unreactive in some assays used to detect such iron in human iron overload. Fractionation by Sephadex G200 chromatography revealed three distinct species with apparent molecular weights of > or = 150 kDa, 40-80 kDa and 1-5 kDa. The iron may be distinguished from known extracellular iron proteins and haem-proteins by its availability to hot acid extractions.

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