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Shungu DC, Bhujwalla ZM, Wehrle JP, et al. 1H NMR spectroscopy of subcutaneous tumors in mice: preliminary studies of effects of growth, chemotherapy and blood flow reduction. NMR Biomed. 1992;5(5):296-302doi: 10.1002/nbm.1940050517.
Shungu, D. C., Bhujwalla, Z. M., Wehrle, J. P., & Glickson, J. D. (1992). 1H NMR spectroscopy of subcutaneous tumors in mice: preliminary studies of effects of growth, chemotherapy and blood flow reduction. NMR in biomedicine, 5(5), 296-302. https://doi.org/10.1002/nbm.1940050517
Shungu, D C, et al. "1H NMR spectroscopy of subcutaneous tumors in mice: preliminary studies of effects of growth, chemotherapy and blood flow reduction." NMR in biomedicine vol. 5,5 (1992): 296-302. doi: https://doi.org/10.1002/nbm.1940050517
Shungu DC, Bhujwalla ZM, Wehrle JP, Glickson JD. 1H NMR spectroscopy of subcutaneous tumors in mice: preliminary studies of effects of growth, chemotherapy and blood flow reduction. NMR Biomed. 1992 Sep-Oct;5(5):296-302. doi: 10.1002/nbm.1940050517. PMID: 1333262.
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NMR Biomed. 1992 Sep-Oct;5(5):296-302. doi: 10.1002/nbm.1940050517.

1H NMR spectroscopy of subcutaneous tumors in mice: preliminary studies of effects of growth, chemotherapy and blood flow reduction.

NMR in biomedicine

D C Shungu, Z M Bhujwalla, J P Wehrle, J D Glickson

Affiliations

  1. Department of Radiology, Johns Hopkins School of Medicine, Baltimore, MD 21205.

PMID: 1333262 DOI: 10.1002/nbm.1940050517

Abstract

This study evaluates a number of methods for obtaining 1H NMR spectra with adequate suppression of lipid and water resonances in two subcutaneously implanted transplantable tumor models (RIF-1 and EMT6/SF). Spin-echo spectra with long TEs (270 ms; water suppressed by presaturation) eliminated lipid resonances from 1H spectra of RIF-1 and decreased lipid contamination in spectra of EMT6/SF; however, spectral sensitivity was substantially reduced. A shorter TE (135 ms) increased sensitivity but did not result in adequate suppression of the lipid peaks. In RIF-1, but not EMT6/SF, adequate lipid suppression was achieved by: (i) spatially selective presaturation of lipid, which in this tumor (but not in EMT6/SF) was localized in a thin region along the periphery of the tumor, followed by a 1-D spin-echo chemical shift imaging pulse sequence (TE = 135 ms); and (ii) 2-D spin-echo chemical shift imaging (TE = 270 ms; approximately 2 x 2 x 9 mm3 voxels). Preliminary 1H studies of the RIF-1 tumor indicate that: (i) there are no significant changes in metabolite levels relative to tumor water during 4 days of untreated tumor growth; (ii) tumor response to chemotherapy with 5-fluorouracil results in a decrease in intensity of all metabolite 1H resonances relative to tumor water, with total choline decreasing the most and lactate the least; and (iii) acute tumor blood flow reduction induced by administration of hydralazine results in doubling of the lactate intensity relative to water.(ABSTRACT TRUNCATED AT 250 WORDS)

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