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Weintraub A, Johnson BN, Stocker BA, et al. Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9. J Bacteriol. 1992;174(6):1916-22doi: 10.1128/jb.174.6.1916-1922.1992.
Weintraub, A., Johnson, B. N., Stocker, B. A., & Lindberg, A. A. (1992). Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9. Journal of bacteriology, 174(6), 1916-22. https://doi.org/10.1128/jb.174.6.1916-1922.1992
Weintraub, A, et al. "Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9." Journal of bacteriology vol. 174,6 (1992): 1916-22. doi: https://doi.org/10.1128/jb.174.6.1916-1922.1992
Weintraub A, Johnson BN, Stocker BA, Lindberg AA. Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9. J Bacteriol. 1992 Mar;174(6):1916-22. doi: 10.1128/jb.174.6.1916-1922.1992. PMID: 1372317; PMCID: PMC205797.
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Atypon Free PMC Article

J Bacteriol. 1992 Mar;174(6):1916-22. doi: 10.1128/jb.174.6.1916-1922.1992.

Structural and immunochemical studies of the lipopolysaccharides of Salmonella strains with both antigen O4 and antigen O9.

Journal of bacteriology

A Weintraub, B N Johnson, B A Stocker, A A Lindberg

Affiliations

  1. Department of Clinical Bacteriology, Huddinge Hospital, Karolinska Institute, Sweden.

PMID: 1372317 PMCID: PMC205797 DOI: 10.1128/jb.174.6.1916-1922.1992
Free PMC Article

Abstract

Two Salmonella hybrid strains, SL5313 (Salmonella typhimurium with a D.rfb+ gene cluster) and SL5396 (S. enteritidis with a B.rfb+ gene cluster), each expressing both O-antigen 4 (of serogroup B) and O-antigen 9 (of serogroup D) were studied by immunofluorescence using a mixture of O4-specific mouse monoclonal and O9-specific rabbit polyclonal antibodies. Bound antibodies, detected by anti-mouse antibody labelled with fluorescein isothiocyanate and anti-rabbit antibody labelled with tetramethylrhodamine isothiocyanate showed that more than 98% of the bacteria expressed both the O4 and O9 epitopes. Phenol-water-extracted lipopolysaccharide from batch-grown cultures subjected to sugar and methylation analyses by gas-liquid chromatography and mass spectrometry were shown to contain abequose (of the O4 epitope) and tyvelose (of the O9 epitope) in ratios of 1:1.5 and 1:2.5 for SL5313 and SL5396, respectively. Isolated polysaccharide chains, obtained by weak-acid hydrolysis of the lipopolysaccharides, were found to contain both O4 and O9 specificities in the same molecule, since polysaccharide bound to O4 antibody attached to a solid-phase-adsorbed O9-specific antibody and vice versa. This demonstrates that in strains SL5313 and SL5396 O chains containing both O4 repeating units (from S. typhimurium) and O9 units (from S. enteritidis) are present.

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