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Gerhardt W, Waldenström J, Gruber W. EDTA effect on creatine kinase (CK) and on the SCE reagent. Scand J Clin Lab Invest. 1979;39(8):737-42doi: 10.1080/00365517909108165.
Gerhardt, W., Waldenström, J., & Gruber, W. (1979). EDTA effect on creatine kinase (CK) and on the SCE reagent. Scandinavian journal of clinical and laboratory investigation, 39(8), 737-42. https://doi.org/10.1080/00365517909108165
Gerhardt, W, et al. "EDTA effect on creatine kinase (CK) and on the SCE reagent." Scandinavian journal of clinical and laboratory investigation vol. 39,8 (1979): 737-42. doi: https://doi.org/10.1080/00365517909108165
Gerhardt W, Waldenström J, Gruber W. EDTA effect on creatine kinase (CK) and on the SCE reagent. Scand J Clin Lab Invest. 1979 Dec;39(8):737-42. doi: 10.1080/00365517909108165. PMID: 119303.
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Scand J Clin Lab Invest. 1979 Dec;39(8):737-42. doi: 10.1080/00365517909108165.

EDTA effect on creatine kinase (CK) and on the SCE reagent.

Scandinavian journal of clinical and laboratory investigation

W Gerhardt, J Waldenström, W Gruber

PMID: 119303 DOI: 10.1080/00365517909108165

Abstract

The inclusion of EDTA in the creatine kinase reagent recommended by the Scandinavian Committee on Enzymes was shown to increase reagent stability from less than 24 h to 5 days. Part of this effect can be explained by the fact that EDTA delays the formation of inhibitory products formed when N-acetyl cysteine is oxidized. The addition of EDTA to the reagent also results in increased measured CK activity. This effect is more pronounced for CK-BB than for CK-MM. Calcium and ferric ions are shown to inhibit the enzyme and the chelation of these ions can partly explain the observed increase of CK acitivity.

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