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Lopaschuk GD, Spafford MA. Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits. Can J Physiol Pharmacol. 1992;70(10):1315-23doi: 10.1139/y92-184.
Lopaschuk, G. D., & Spafford, M. A. (1992). Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits. Canadian journal of physiology and pharmacology, 70(10), 1315-23. https://doi.org/10.1139/y92-184
Lopaschuk, G D, and Spafford, M A. "Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits." Canadian journal of physiology and pharmacology vol. 70,10 (1992): 1315-23. doi: https://doi.org/10.1139/y92-184
Lopaschuk GD, Spafford MA. Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits. Can J Physiol Pharmacol. 1992 Oct;70(10):1315-23. doi: 10.1139/y92-184. PMID: 1490250.
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Can J Physiol Pharmacol. 1992 Oct;70(10):1315-23. doi: 10.1139/y92-184.

Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits.

Canadian journal of physiology and pharmacology

G D Lopaschuk, M A Spafford

Affiliations

  1. Department of Pediatrics, University of Alberta, Edmonton, Canada.

PMID: 1490250 DOI: 10.1139/y92-184

Abstract

Between 1 and 7 days of life, the newborn rabbit heart shifts from predominantly using carbohydrates to predominantly using fatty acids as an energy substrate. We therefore used isolated working hearts from 1- or 7-day-old rabbits to determine the effects of fatty acids on myocardial glucose use and the ability of hearts to recover following various periods of transient no-flow ischemia. One-day-old hearts were perfused via the inferior vena cava and ejected buffer through the cannulated aorta and pulmonary artery. Seven-day-old hearts were perfused via the left atrium and ejected buffer through the cannulated aorta. To measure glucose use, hearts were perfused with 11 mM [3H, 14C]glucose, 3% albumin, and 500 microU insulin/mL, in the presence or absence of 0.4 mM palmitate. In the absence of fatty acids, glycolytic rates were similar in 1- and 7-day-old hearts, whereas glucose oxidation rates were 5 times greater in 7-day-old hearts. Palmitate did not have any major effects on overall glucose use in 1-day-old hearts, but did markedly inhibit glycolysis and glucose oxidation in 7-day-old hearts. A series of hearts were also subjected to periods (25-60 min) of no-flow ischemia, followed by 30 min of aerobic reperfusion. In the absence of palmitate, 1-day-old hearts subjected to ischemic periods of up to 60 min recovered some degree of mechanical function during reperfusion, whereas 7-day-old rabbit hearts failed to recover if hearts were subjected to ischemic periods of 35 min or longer.(ABSTRACT TRUNCATED AT 250 WORDS)

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