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Sekido Y, Takahashi T, Mäkelä TP, et al. Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer. Mol Cell Biol. 1992;12(4):1747-54doi: 10.1128/mcb.12.4.1747-1754.1992.
Sekido, Y., Takahashi, T., Mäkelä, T. P., Obata, Y., Ueda, R., Hida, T., Hibi, K., Shimokata, K., Alitalo, K., & Takahashi, T. (1992). Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer. Molecular and cellular biology, 12(4), 1747-54. https://doi.org/10.1128/mcb.12.4.1747-1754.1992
Sekido, Y, et al. "Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer." Molecular and cellular biology vol. 12,4 (1992): 1747-54. doi: https://doi.org/10.1128/mcb.12.4.1747-1754.1992
Sekido Y, Takahashi T, Mäkelä TP, Obata Y, Ueda R, Hida T, Hibi K, Shimokata K, Alitalo K, Takahashi T. Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer. Mol Cell Biol. 1992 Apr;12(4):1747-54. doi: 10.1128/mcb.12.4.1747-1754.1992. PMID: 1312669; PMCID: PMC369618.
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Atypon Free PMC Article

Mol Cell Biol. 1992 Apr;12(4):1747-54. doi: 10.1128/mcb.12.4.1747-1754.1992.

Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small-cell lung cancer.

Molecular and cellular biology

Y Sekido, T Takahashi, T P Mäkelä, Y Obata, R Ueda, T Hida, K Hibi, K Shimokata, K Alitalo, T Takahashi

Affiliations

  1. First Department of Internal Medicine, Nagoya University School of Medicine, Japan.

PMID: 1312669 PMCID: PMC369618 DOI: 10.1128/mcb.12.4.1747-1754.1992
Free PMC Article

Abstract

The L-myc gene was first isolated from a human small-cell lung cancer (SCLC) cell line on the basis of its amplification and sequence similarity to c-myc and N-myc. A new mechanism of L-myc activation which results from the production of rlf-L-myc fusion protein was recently reported. On the basis of our earlier observation of a rearrangement involving amplified L-myc in an SCLC cell line, ACC-LC-49, we decided to investigate this rearrangement in detail along with the structure of L-myc amplification units in five additional SCLC cell lines. We report here the identification of a novel genomic region, termed jal, which is distinct from rlf and is juxtaposed to and amplified with L-myc during the process of DNA amplification of the region encompassing L-myc. Long-range analysis using pulsed-field gel electrophoresis revealed that the amplified L-myc locus is involved in highly complex intrachromosomal rearrangements with jal and/or rlf. Our results also suggest that the simultaneous presence of rearrangements both in rlf intron 1 and in regions immediately upstream of L-myc may be necessary for the expression of rlf-L-myc chimeric transcripts.

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