J Biol Chem. 1992 Nov 25;267(33):23502-6.

Regulation of integrin gene expression by substrate adherence.

The Journal of biological chemistry

D Chen, V Magnuson, S Hill, C Arnaud, B Steffensen, R J Klebe

PMID: 1429694
Free Article

Abstract

Under substrate adherent conditions, integrin gene expression can be regulated by transforming growth factor-beta, interleukin-1 beta, and prostaglandins. This report demonstrates a new mechanism that can differentially control the expression of several integrins. When MG-63 osteosarcoma cells are maintained in suspension, up-regulation of several integrin alpha-subunits takes place. Within as little as 4 h, the mRNA levels for both the alpha 2- and alpha 4-subunits are increased 4- and 6-fold, respectively. It was found that mRNA levels for the alpha 2-, alpha 4-, and alpha v-subunits were markedly increased in several differentiated cell lines under nonadherent conditions; however, cells that did not express a given integrin under substrate adherent conditions also did not express this integrin when maintained in suspension. The alpha 5-subunit did not upregulate during suspension growth. By immunocytochemistry, changes in integrin mRNA levels were confirmed at the protein level. Both cytochalasin B and a phorbol ester were found to induce the expression of the alpha 2-subunit, but not the alpha 4- and alpha 5-subunits, in a dose-dependent fashion. Many investigators have documented changes in gene expression that result from changes in "cell shape." These phenomena may result from up-regulation of integrin gene expression induced by the lack of substrate adherence.

Substances

• Integrins
• Macromolecular Substances
• Oligodeoxyribonucleotides
• RNA, Messenger
• Cytochalasin B
• Tetradecanoylphorbol Acetate

MeSH terms

• Base Sequence
• Cell Adhesion*
• Cell Division
• Cell Line
• Cytochalasin B / pharmacology
• Gene Expression Regulation* / drug effects
• Gene Expression Regulation, Neoplastic* / drug effects
• Humans
• Integrins / genetics
• Kinetics
• Macromolecular Substances
• Molecular Sequence Data
• Oligodeoxyribonucleotides
• Polymerase Chain Reaction / methods
• RNA, Messenger / genetics
• RNA, Messenger / metabolism
• Tetradecanoylphorbol Acetate / pharmacology
• Tumor Cells, Cultured

Publication Types

• Journal Article
• Research Support, U.S. Gov't, Non-P.H.S.
• Research Support, U.S. Gov't, P.H.S.

Grant support

• DE08144/DE/NIDCR NIH HHS/United States

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