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Elsevier Science

Carbohydr Res. 1992 Jul 02;231:1-11. doi: 10.1016/0008-6215(92)84002-a.

The structure of the O-specific polysaccharide chain of the lipopolysaccharide of Salmonella arizonae O61.

Carbohydrate research

E V Vinogradov, A S Shashkov, Y A Knirel, N K Kochetkov, J Dabrowski, H Grosskurth, E S Stanislavsky, E V Kholodkova

Affiliations

  1. N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.

PMID: 1394306 DOI: 10.1016/0008-6215(92)84002-a

Abstract

The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-nonulosonic acid (Sug). On the basis of partial acid hydrolysis with 0.1 M HCl, solvolysis with anhydrous HF in methanol, and 1H- and 13C-NMR analysis (including 1H/13C inversely correlated spectroscopy for localisation of N-acyl substituents), it was concluded that the O-specific polysaccharide had the following structure. ----3)-alpha-L-FucAm-(1----3)-alpha-D-GlcNAc-(1----8)-beta-Sug+ ++-(2---- The O-antigen of S. arizonae O61 is structurally related to that of Pseudomonas aeruginosa O12, thus explaining the known serological cross-reactivity between these micro-organisms.

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