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Boisset N, Pochon F, Chwetzoff S, et al. Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand. J Struct Biol. 1992;108(3):221-6doi: 10.1016/1047-8477(92)90022-3.
Boisset, N., Pochon, F., Chwetzoff, S., Barray, M., Delain, E., & Lamy, J. (1992). Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand. Journal of structural biology, 108(3), 221-6. https://doi.org/10.1016/1047-8477(92)90022-3
Boisset, N, et al. "Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand." Journal of structural biology vol. 108,3 (1992): 221-6. doi: https://doi.org/10.1016/1047-8477(92)90022-3
Boisset N, Pochon F, Chwetzoff S, Barray M, Delain E, Lamy J. Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand. J Struct Biol. 1992 May-Jun;108(3):221-6. doi: 10.1016/1047-8477(92)90022-3. PMID: 1282356.
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J Struct Biol. 1992 May-Jun;108(3):221-6. doi: 10.1016/1047-8477(92)90022-3.

Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand.

Journal of structural biology

N Boisset, F Pochon, S Chwetzoff, M Barray, E Delain, J Lamy

Affiliations

  1. Laboratoire de Biochimie Fondamentale, Université François Rabelais, CNRS URA 1334, Tours, France.

PMID: 1282356 DOI: 10.1016/1047-8477(92)90022-3

Abstract

In order to covalently bind the hydrolyzed thiol ester groups of the human alpha 2-macroglobulin (alpha 2M) transformed by methylamine, the phospholipase A2 (PLA2), a small enzyme (M(r) = 13,000) from Naja nigricollis snake venom was activated by succinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate (SMCC). Average images determined from electron micrographs of the methylamine-transformed alpha 2M, with and without activated PLA2, were determined by image processing and compared. A localization of the PLA2 was achieved by subtracting the average image of alpha 2M transformed by methylamine from that containing PLA2. The results are consistent with previous work showing the central localization of chymotrypsin trapped in alpha 2M. They also suggest that the four thiol esters are located near the center of the alpha 2M molecule.

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