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McCauley LK, Rosol TJ, Merryman JI, et al. Parathyroid hormone-related protein binding to human T-cell lymphotropic virus type I-infected lymphocytes. Endocrinology. 1992;130(1):300-6doi: 10.1210/endo.130.1.1309334.
McCauley, L. K., Rosol, T. J., Merryman, J. I., & Capen, C. C. (1992). Parathyroid hormone-related protein binding to human T-cell lymphotropic virus type I-infected lymphocytes. Endocrinology, 130(1), 300-6. https://doi.org/10.1210/endo.130.1.1309334
McCauley, L K, et al. "Parathyroid hormone-related protein binding to human T-cell lymphotropic virus type I-infected lymphocytes." Endocrinology vol. 130,1 (1992): 300-6. doi: https://doi.org/10.1210/endo.130.1.1309334
McCauley LK, Rosol TJ, Merryman JI, Capen CC. Parathyroid hormone-related protein binding to human T-cell lymphotropic virus type I-infected lymphocytes. Endocrinology. 1992 Jan;130(1):300-6. doi: 10.1210/endo.130.1.1309334. PMID: 1309334.
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Endocrinology. 1992 Jan;130(1):300-6. doi: 10.1210/endo.130.1.1309334.

Parathyroid hormone-related protein binding to human T-cell lymphotropic virus type I-infected lymphocytes.

Endocrinology

L K McCauley, T J Rosol, J I Merryman, C C Capen

Affiliations

  1. Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.

PMID: 1309334 DOI: 10.1210/endo.130.1.1309334

Abstract

An HTLV-I-infected human lymphocyte line (MT-2) was evaluated for 1) the presence of receptors for PTH-related protein (PTHrP), 2) cell proliferation in response to PTHrP, and 3) adrenylate cyclase and intracellular calcium response to PTHrP. PTHrP-(1-36) was labeled with 125I, purified, and used to detect binding to MT-2 cells. Specific binding ranged between 4-9% of the total radioactivity. Specific binding increased with increasing cell number, was maximal within 30-60 min, and was highest at 37 C. Scatchard analysis revealed a one-binding site fit, with a Kd of 14.5 nM. Binding was not competed for by calcitonin, calcitonin gene-related peptide, or interleukin-1 beta. PTHrP at 1.0 and 0.1 microM inhibited proliferation in MT-2 cells. PTHrP did not alter adenylate cyclase stimulation in MT-2 cells, but did cause an increase in intracellular calcium. These findings indicate that MT-2 cells have receptors for PTHrP and are consistent with a potential autocrine role of PTHrP in HTLV-I-infected lymphoid cells.

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