Exp Hematol. 1992 Oct;20(9):1055-60.

Vanadate mimics the effect of stem cell factor on highly purified human erythroid burst-forming units in vitro, but not the effect of erythropoietin.

Experimental hematology

C H Dai, S Krantz

PMID: 1281783

Abstract

When orthovanadate, an inhibitor of protein tyrosine phosphatase activity, was added to highly purified human blood erythroid burst-forming units (BFU-E) a marked increase in the number and size of erythroid bursts was evident at an optimum concentration of 4 microM. Because BFU-E are stimulated by stem cell factor (SCF), interleukin 3 (IL-3), and erythropoietin (EP), this effect could occur through an enhancement of any one of these pathways. However, no effect was observed on human erythroid colony-forming units (CFU-E), indicating that vanadate was not potentiating the effect of EP. The time course of decline in BFU-E, when vanadate was added in vitro on successive days, followed the time course produced by delayed addition of SCF but not IL-3. In addition, vanadate markedly enhanced the effect of an optimal concentration of IL-3, but it could only enhance the effect of SCF when SCF concentrations were less than optimum. These experiments demonstrate that vanadate markedly stimulates the number and size of human BFU-E in vitro and that it mimics the effect of SCF. Vanadate may be acting as a phosphatase inhibitor that potentiates the kinase activity induced by SCF, but elucidation of its specific biochemical effects on these cells awaits further investigation.

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Substances

• Hematopoietic Cell Growth Factors
• Interleukin-3
• Stem Cell Factor
• Erythropoietin
• Vanadates

MeSH terms

• Cells, Cultured
• Dose-Response Relationship, Drug
• Erythroid Precursor Cells / cytology
• Erythroid Precursor Cells / drug effects
• Erythropoiesis / drug effects
• Erythropoiesis / physiology
• Erythropoietin / pharmacology
• Hematopoietic Cell Growth Factors / pharmacology
• Humans
• Interleukin-3 / pharmacology
• Stem Cell Factor
• Time Factors
• Vanadates / pharmacology

Publication Types

• Comparative Study
• Journal Article
• Research Support, Non-U.S. Gov't
• Research Support, U.S. Gov't, Non-P.H.S.
• Research Support, U.S. Gov't, P.H.S.

Grant support

• DK-15555/DK/NIDDK NIH HHS/United States
• T32 DK07186/DK/NIDDK NIH HHS/United States

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