Display options
Cite
McCray JA, Fidler-Lim N, Ellis-Davies GC, et al. Rate of release of Ca2+ following laser photolysis of the DM-nitrophen-Ca2+ complex. Biochemistry. 1992;31(37):8856-61doi: 10.1021/bi00152a023.
McCray, J. A., Fidler-Lim, N., Ellis-Davies, G. C., & Kaplan, J. H. (1992). Rate of release of Ca2+ following laser photolysis of the DM-nitrophen-Ca2+ complex. Biochemistry, 31(37), 8856-61. https://doi.org/10.1021/bi00152a023
McCray, J A, et al. "Rate of release of Ca2+ following laser photolysis of the DM-nitrophen-Ca2+ complex." Biochemistry vol. 31,37 (1992): 8856-61. doi: https://doi.org/10.1021/bi00152a023
McCray JA, Fidler-Lim N, Ellis-Davies GC, Kaplan JH. Rate of release of Ca2+ following laser photolysis of the DM-nitrophen-Ca2+ complex. Biochemistry. 1992 Sep 22;31(37):8856-61. doi: 10.1021/bi00152a023. PMID: 1390672.
Copy Download .nbib Format: NLM
Share it on

Biochemistry. 1992 Sep 22;31(37):8856-61. doi: 10.1021/bi00152a023.

Rate of release of Ca2+ following laser photolysis of the DM-nitrophen-Ca2+ complex.

Biochemistry

J A McCray, N Fidler-Lim, G C Ellis-Davies, J H Kaplan

Affiliations

  1. Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085.

PMID: 1390672 DOI: 10.1021/bi00152a023

Abstract

The determination of the rate of release of Ca2+ by pulsed photolysis of the photolabile chelator DM-nitrophen is important for its use in time-resolved physiological studies: the rate of substrate or effector release should be faster than the processes they initiate. Flash photolysis of DM-nitrophen using a 50-ns pulse from a frequency-doubled ruby laser (with emission at 347 nm having energy of ca. 10-20 mJ) yields short-lived photochromic or aci-nitro intermediates. At pH 6.9, double-exponential decay of a photochromic intermediate was observed for DM-nitrophen itself and its Ca2+ complex (tau 1/2 values of 24 and 570 microseconds, and 32 and 220 microseconds respectively), while only monoexponential decay of the DM-nitrophen-Mg2+ complex was detected (tau 1/2 = 31 microseconds). Only the photochemistry of DM-nitrophen-Ca2+ was found to be pH sensitive (monoexponential decay, tau 1/2 approximately 115 microseconds at pH 7.9 and 8.9). Use of the Ca(2+)-sensitive metallochromic dye antipyrylazo III in conjunction with pulsed photolysis of DM-nitrophen-Ca2+ enabled an upper limit of the half-time of release of Ca2+ to be established of ca. 180 microseconds (the rate of association of Ca2+ with the dye was probably rate determining). The rate of Ca2+ photorelease may, however, be faster than this. Thus, the DM-nitrophen-Ca2+ complex releases Ca2+ on photolysis sufficiently rapidly for the study of many Ca(2+)-dependent physiological processes with improved kinetic resolution over conventional mixing methods.

Similar articles

Glebov EM, Matveeva SG, Pozdnyakov IP, Grivin VP, Plyusnin VF, Vasilchenko DB, Romanova TE, Melnikov AA, Chekalin SV, Fedunov RG.
Photochem Photobiol Sci. 2020 Nov 11;19(11):1569-1579. doi: 10.1039/d0pp00244e.
PMID: 33073834

Riordan M, Zajc WA.
Sci Am. 2006 May;294(5):34A, 35-41. doi: 10.1038/scientificamerican0506-34a.
PMID: 16708486

Cited by

Substances

MeSH terms

Publication Types

Grant support

LinkOut - more resources