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Olobo JO, Reid GD, Githure JI, et al. IFN-gamma and delayed-type hypersensitivity are associated with cutaneous leishmaniasis in vervet monkeys following secondary rechallenge with Leishmania major. Scand J Immunol Suppl. 1992;11:48-52doi: 10.1111/j.1365-3083.1992.tb01618.x.
Olobo, J. O., Reid, G. D., Githure, J. I., & Anjili, C. O. (1992). IFN-gamma and delayed-type hypersensitivity are associated with cutaneous leishmaniasis in vervet monkeys following secondary rechallenge with Leishmania major. Scandinavian journal of immunology. Supplement, 1148-52. https://doi.org/10.1111/j.1365-3083.1992.tb01618.x
Olobo, J O, et al. "IFN-gamma and delayed-type hypersensitivity are associated with cutaneous leishmaniasis in vervet monkeys following secondary rechallenge with Leishmania major." Scandinavian journal of immunology. Supplement vol. 11 (1992): 48-52. doi: https://doi.org/10.1111/j.1365-3083.1992.tb01618.x
Olobo JO, Reid GD, Githure JI, Anjili CO. IFN-gamma and delayed-type hypersensitivity are associated with cutaneous leishmaniasis in vervet monkeys following secondary rechallenge with Leishmania major. Scand J Immunol Suppl. 1992;11:48-52. doi: 10.1111/j.1365-3083.1992.tb01618.x. PMID: 1514049.
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Scand J Immunol Suppl. 1992;11:48-52. doi: 10.1111/j.1365-3083.1992.tb01618.x.

IFN-gamma and delayed-type hypersensitivity are associated with cutaneous leishmaniasis in vervet monkeys following secondary rechallenge with Leishmania major.

Scandinavian journal of immunology. Supplement

J O Olobo, G D Reid, J I Githure, C O Anjili

Affiliations

  1. Institute of Primate Research, National Museums of Kenya, Nairobi.

PMID: 1514049 DOI: 10.1111/j.1365-3083.1992.tb01618.x

Abstract

IFN-gamma levels and delayed-type hypersensitivity (DTH) responses were evaluated in vervet monkeys, following secondary infection with Leishmania major (L. major). The animals had previously been vaccinated with leishmanial antigen, exposed to a primary infection and allowed to self-cure. Supernatants of peripheral blood mononuclear cell (PBMC) cultures, stimulated with either L. major antigen or Concanavalin A (Con A), were examined for the presence of IFN-gamma in a double sandwich enzyme-linked immunosorbent assay (ELISA). Significant levels of IFN-gamma were detected during active disease and following self-cure in both antigen and Con A supernatants. Higher levels of IFN-gamma were, however, present during active disease as compared with after self-cure. Positive and strong DTH responses were elicited in all experimental animals, following intradermal injection of fixed promastigotes (5 x 10(7)/animal) before rechallenge, during active infection and following self-cure. Again, strongest DTH responses were obtained during active infection as compared with the other sampling points. There was a correlation between IFN-gamma levels and DTH responses. It was concluded that IFN-gamma secretion and positive DTH responses are associated with secondary L. major infection and represent specific immunological correlates of protection in this disease model.

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