Display options
Cite
Defoort JP, Nardelli B, Huang W, et al. A rational design of synthetic peptide vaccine with a built-in adjuvant. A modular approach for unambiguity. Int J Pept Protein Res. 1992;40(3):214-21doi: 10.1111/j.1399-3011.1992.tb00294.x.
Defoort, J. P., Nardelli, B., Huang, W., & Tam, J. P. (1992). A rational design of synthetic peptide vaccine with a built-in adjuvant. A modular approach for unambiguity. International journal of peptide and protein research, 40(3), 214-21. https://doi.org/10.1111/j.1399-3011.1992.tb00294.x
Defoort, J P, et al. "A rational design of synthetic peptide vaccine with a built-in adjuvant. A modular approach for unambiguity." International journal of peptide and protein research vol. 40,3 (1992): 214-21. doi: https://doi.org/10.1111/j.1399-3011.1992.tb00294.x
Defoort JP, Nardelli B, Huang W, Tam JP. A rational design of synthetic peptide vaccine with a built-in adjuvant. A modular approach for unambiguity. Int J Pept Protein Res. 1992 Sep-Oct;40(3):214-21. doi: 10.1111/j.1399-3011.1992.tb00294.x. PMID: 1478779.
Copy Download .nbib Format: NLM
Share it on

Int J Pept Protein Res. 1992 Sep-Oct;40(3):214-21. doi: 10.1111/j.1399-3011.1992.tb00294.x.

A rational design of synthetic peptide vaccine with a built-in adjuvant. A modular approach for unambiguity.

International journal of peptide and protein research

J P Defoort, B Nardelli, W Huang, J P Tam

Affiliations

  1. Rockefeller University, New York, NY.

PMID: 1478779 DOI: 10.1111/j.1399-3011.1992.tb00294.x

Abstract

We describe a peptide vaccine model containing a built-in adjuvant. This model used a multiple antigen peptide system (MAPS) to amplify peptide antigens and a lipoamino acid, tripalmitoyl glyceryl cysteine (P3C), as a built-in adjuvant. An 18-residue peptide antigen (B2) derived from the third variable domain (amino acid 312-329) of the glycoprotein gp120 of type I human immunodeficiency virus (HIV-1) was used in this model. This peptide antigen is a suitable target since it consists of neutralizing, T-helper, and T-cytotoxic epitopes. The peptide antigen in a tetravalent MAPS format (B2M-P3C) with a lipophilic attachment was synthesized by two routes for comparison: a direct stepwise approach and an indirect modular approach. In the stepwise approach, each residue was sequentially added to the peptide resin to give B2M-P3C and the P3C was incorporated to the side chain of a carboxyl terminal lysine as Fmoc-Lys(P3C). In the modular approach, a module containing a chloroacetylated core matrix of MAPS (M-P3C) with a carboxyl tetrapeptide bearing Lys(P3C) and a second module containing the peptide antigen B2 with a cysteine at its terminus were synthesized and purified separately, and then coupled to each other to form B2M-P3C. In the modular approach, the molecular ion of B2M-P3C was unambiguously identified by ion-spray mass spectrometry. B2M-P3C, administered in liposomes without any adjuvant such as Freund's complete adjuvant, was used to immunize mice and found to induce gp120-specific antibodies in vitro, and prime cytotoxic T lymphocytes in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)

Cited by

Substances

MeSH terms

Publication Types

Grant support

LinkOut - more resources