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Biotechniques. 1992 Oct;13(4):540-3.

Nonradioactive southwestern analysis using chemiluminescent detection.

BioTechniques

S Dooley, C Welter, N Blin

Affiliations

  1. Institute of Human Genetics, University of Saarland, Homburg, FRG.

PMID: 1476722

Abstract

Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.

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