Blood. 1992 Sep 01;80(5):1218-23.

Interleukin-11 stimulates multiple phases of erythropoiesis in vitro.

Blood

V F Quesniaux, S C Clark, K Turner, B Fagg

PMID: 1381240

Abstract

Interleukin-11 (IL-11), a pleiotropic cytokine originally isolated from a primate bone marrow stromal cell line, has been shown to stimulate T-cell-dependent B-cell maturation, megakaryopoiesis, and various stages of myeloid differentiation, but to inhibit adipogenesis. Because stromal cells are essential for the maintenance of early hematopoietic progenitor cells in long-term culture, we investigated the effects of IL-11 on multipotent and erythroid precursors from murine bone marrow in vitro in suspension and semisolid cultures. Our results show that in the presence of IL-3 or c-kit ligand (KL), IL-11 has profound stimulatory effects on primitive multilineage hematopoietic progenitors, pre-CFC(multi), as well as on precursors representing various stages of erythroid differentiation observable in vitro, including CFC(multi), BFU-E, and CFU-E. In addition, the combination of KL with IL-11 also stimulated highly proliferative erythroid progenitors that yield remarkable macroscopic erythroblast colonies in culture. These results indicate that IL-11 is likely to play a pivotal role in early hematopoiesis and at multiple stages of erythropoiesis.

Substances

• Hematopoietic Cell Growth Factors
• Interleukin-11
• Interleukin-3
• Interleukins
• Stem Cell Factor
• Erythropoietin

MeSH terms

• Animals
• Erythroid Precursor Cells / drug effects
• Erythroid Precursor Cells / physiology
• Erythropoiesis / drug effects
• Erythropoietin / pharmacology
• Hematopoietic Cell Growth Factors / pharmacology
• In Vitro Techniques
• Interleukin-11
• Interleukin-3 / pharmacology
• Interleukins / pharmacology
• Mice
• Stem Cell Factor

Publication Types

• Journal Article

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