Display options
Cite
Hatai M, Takahara K, Hashi H, et al. Effects of fibronectin-type V collagen recombinant fusion protein on cell adhesion and cell proliferation. Cell Struct Funct. 1992;17(5):293-300doi: 10.1247/csf.17.293.
Hatai, M., Takahara, K., Hashi, H., Kato, I., & Yaoi, Y. (1992). Effects of fibronectin-type V collagen recombinant fusion protein on cell adhesion and cell proliferation. Cell structure and function, 17(5), 293-300. https://doi.org/10.1247/csf.17.293
Hatai, M, et al. "Effects of fibronectin-type V collagen recombinant fusion protein on cell adhesion and cell proliferation." Cell structure and function vol. 17,5 (1992): 293-300. doi: https://doi.org/10.1247/csf.17.293
Hatai M, Takahara K, Hashi H, Kato I, Yaoi Y. Effects of fibronectin-type V collagen recombinant fusion protein on cell adhesion and cell proliferation. Cell Struct Funct. 1992 Oct;17(5):293-300. doi: 10.1247/csf.17.293. PMID: 1473159.
Copy Download .nbib Format: NLM
Share it on
Full text links
J-STAGE, Japan Science and Technology Information Aggregator, Electronic

Cell Struct Funct. 1992 Oct;17(5):293-300. doi: 10.1247/csf.17.293.

Effects of fibronectin-type V collagen recombinant fusion protein on cell adhesion and cell proliferation.

Cell structure and function

M Hatai, K Takahara, H Hashi, I Kato, Y Yaoi

Affiliations

  1. Biology Division, National Cancer Center Research Institute, Tokyo, Japan.

PMID: 1473159 DOI: 10.1247/csf.17.293
Free Article

Abstract

An expression vector pTF7520-Col-V-In, which encodes a fusion protein of the cell-binding domain of fibronectin (C277) and the insulin- and heparin-binding domain of the alpha 1 chain of human type V collagen, was constructed. E. coli transfected with this plasmid synthesized a 50-kDa fusion protein. This fusion protein, C277-V, was purified from the crude extract by a single step heparin HPLC. Similar amounts of insulin bound to purified C277-V and to the alpha 1 chain of type V collagen as judged by the binding of peroxidase-conjugated insulin. Cell-adhesive activity of C277-V was lower than that of the original fibronectin fragment C274, but similar numbers of cells adhered to both protein substrates when the culture dishes were coated with 1 mM of each protein. Insulin bound to the C277-V substratum stimulated the growth of mouse mammary tumor MTD cells in serum-free culture medium.

Substances

MeSH terms

Publication Types

LinkOut - more resources