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Yang Y, Bu D, Wang K, et al. Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein. Beijing Da Xue Xue Bao Yi Xue Ban. 2003;35(4):426-8
Yang, Y., Bu, D., Wang, K., Tu, P., & Zhu, X. (2003). Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein. Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences, 35(4), 426-8.
Yang, Yong, et al. "Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein." Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences vol. 35,4 (2003): 426-8.
Yang Y, Bu D, Wang K, Tu P, Zhu X. Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein. Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Aug;35(4):426-8. PMID: 12947563.
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Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Aug;35(4):426-8.

Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein.

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences

Yong Yang, Dingfang Bu, Ke Wang, Ping Tu, Xuejun Zhu

Affiliations

  1. Department of Dermatology, Peking University First Hospital, Beijing 100034, China. [email protected]

PMID: 12947563

Abstract

OBJECTIVE: To clone and express xeroderma pigmentosum, complementation group A (XPA) cDNA and to identify its recombinant protein.

METHODS: Coding region of XPA cDNA was amplified from human tonsil cDNA by reverse transcription and nested PCR. The PCR product was cloned into pBluescript vector, confirmed by DNA sequencing, cloned in frame into a prokaryotic expression vector pTrcHis C and expressed in E. coli. TOP10. The recombinant fusion protein was identified by immunoblotting.

RESULTS: The entire coding region of XPA cDNA was cloned and expressed. The fusion XPA protein was identified by anti-XPA monoclonal antibody on western blot.

CONCLUSION: Cloning of human XPA cDNA and successful expression of recombinant XPA protein will be useful for the construction of a viral gene transfer vector for the gene therapy of XPA.

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