Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Jun;17(2):162-4.

[Polymorphism of CXCR4 coding region of human immunodeficiency virus-1 in Chinese Han people].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology

[Article in Chinese]
Ming-xu Liu, Fu-sheng Wang, Wei-guo Hong, Bo Wang, Lei Jin, Zhou-yun Lei, Jing Hou

PMID: 12870001

Abstract

OBJECTIVE: To study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.

METHODS: Totally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers. The results of the same sequencing primers were analyzed by DNAstar software to find and identify single nucleotide polymorphism (SNP) sites.

RESULTS: Totally 7 SNPs were found in the coding region of CXCR4, among them 3 were synonymous mutation (C-->T at loci 129, 426 and 968), 3 were missense mutation (C-->T at locus 38, A-->T at locus 90, and A-->C at locus 712) and 1 was stop mutation (C-->T at 106, which converted the codon for glutamic acid into stop codon).

CONCLUSIONS: The polymorphism of CXCR4 coding region in Chinese Han is probably different from that of the other ethnic groups. Six of the 7 SNPs were discovered for the first time. Their influences on AIDS progression are worthy of studying.

Substances

• Receptors, CXCR4

MeSH terms

• Adult
• Asians
• Base Sequence
• China / ethnology
• Female
• Gene Frequency
• HIV-1 / genetics
• Humans
• Male
• Middle Aged
• Molecular Sequence Data
• Point Mutation*
• Polymorphism, Single Nucleotide
• Receptors, CXCR4 / genetics

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't