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Zhou QH, Gong YL, Qin Y, et al. [Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients]. Zhonghua Zhong Liu Za Zhi. 2003;25(1):62-5
Zhou, Q. H., Gong, Y. L., Qin, Y., Sun, Z. L., Sun, Z. F., Liu, L. X., Li, L., & Zhu, W. (2003). [Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients]. Zhonghua zhong liu za zhi [Chinese journal of oncology], 25(1), 62-5.
Zhou, Qing-hua, et al. "[Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients]." Zhonghua zhong liu za zhi [Chinese journal of oncology] vol. 25,1 (2003): 62-5.
Zhou QH, Gong YL, Qin Y, Sun ZL, Sun ZF, Liu LX, Li L, Zhu W. [Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients]. Zhonghua Zhong Liu Za Zhi. 2003 Jan;25(1):62-5. Chinese. PMID: 12678991.
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Zhonghua Zhong Liu Za Zhi. 2003 Jan;25(1):62-5.

[Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients].

Zhonghua zhong liu za zhi [Chinese journal of oncology]

[Article in Chinese]
Qing-hua Zhou, You-ling Gong, Yang Qin, Zhi-lin Sun, Ze-fang Sun, Lun-xu Liu, Lu Li, Wen Zhu

Affiliations

  1. Laboratory of Lung Cancer Molecular Biology, Cancer Center and Cancer Institute, West China Hospital, Sichuan University, Chengdu 610041, China.

PMID: 12678991

Abstract

OBJECTIVE: To evaluate the specificity, sensitivity and their clinical significance of detecting CK19 mRNA expression by nested RT-PCR for molecular diagnosis of micrometastasis in the peripheral blood and bone marrow of patients with non-small cell lung cancer.

METHODS: CK19 mRNA expression was detected by nested RT-PCR in peripheral blood and bone marrow samples from 59 lung cancer patients, with samples of 11 benign pulmonary lesion patients and 20 healthy adults as control.

RESULTS: The sensitivity of nested RT-PCR was 10(-6). The positive rates of micrometastasis were 33.89% (20/59) in peripheral blood and 22.03% (13/59) in bone marrow, with a highly positive correlation existing between the two groups (P < 0.05). The micrometastasis in peripheral blood and bone marrow was closely correlated with the pathological classification and cell differentiation (P < 0.05) and P-TNM stage (P < 0.01). No CK19 mRNA expression was found in the samples from patients with benign pulmonary lesion or healthy adult volunteers.

CONCLUSION: The peripheral blood and bone marrow from patients with non-small cell lung cancer possesses micrometastasis that can not be detected by common methods. Nested RT-PCR technique shows favorable specificity and sensitivity in detecting the condition with definite clinical prospects.

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