Display options
Cite
Walsh DA, Mapp PI, Wharton J, et al. Localisation and characterisation of substance P binding to human synovial tissue in rheumatoid arthritis. Ann Rheum Dis. 1992;51(3):313-7doi: 10.1136/ard.51.3.313.
Walsh, D. A., Mapp, P. I., Wharton, J., Rutherford, R. A., Kidd, B. L., Revell, P. A., Blake, D. R., & Polak, J. M. (1992). Localisation and characterisation of substance P binding to human synovial tissue in rheumatoid arthritis. Annals of the rheumatic diseases, 51(3), 313-7. https://doi.org/10.1136/ard.51.3.313
Walsh, D A, et al. "Localisation and characterisation of substance P binding to human synovial tissue in rheumatoid arthritis." Annals of the rheumatic diseases vol. 51,3 (1992): 313-7. doi: https://doi.org/10.1136/ard.51.3.313
Walsh DA, Mapp PI, Wharton J, Rutherford RA, Kidd BL, Revell PA, Blake DR, Polak JM. Localisation and characterisation of substance P binding to human synovial tissue in rheumatoid arthritis. Ann Rheum Dis. 1992 Mar;51(3):313-7. doi: 10.1136/ard.51.3.313. PMID: 1374227; PMCID: PMC1004650.
Copy Download .nbib Format: NLM
Share it on
Full text links
HighWire Free PMC Article

Ann Rheum Dis. 1992 Mar;51(3):313-7. doi: 10.1136/ard.51.3.313.

Localisation and characterisation of substance P binding to human synovial tissue in rheumatoid arthritis.

Annals of the rheumatic diseases

D A Walsh, P I Mapp, J Wharton, R A Rutherford, B L Kidd, P A Revell, D R Blake, J M Polak

Affiliations

  1. Inflammation Group, London Hospital, Whitechapel, United Kingdom.

PMID: 1374227 PMCID: PMC1004650 DOI: 10.1136/ard.51.3.313
Free PMC Article

Abstract

The neuropeptide substance P is found in perivascular and free unmyelinated nerve fibres in human synovial tissue. Quantitative receptor autoradiography was used to show specific, high affinity (Kd = 0.75 (0.21), nmol/l (mean (standard error of the mean)), low capacity (Bmax = 27.8 (7.9) amol/mm2) binding sites for substance P Bolton Hunter-labelled with iodine-125 localised to vascular endothelial cells in human synovial tissue. The binding could be saturated, was reversible, and was dependent on the magnesium concentration. Unlabelled substance P and neurokinin A competitively inhibited specific binding with 50% inhibition at concentrations of 1.25 (0.21) and 175 (29) nmol/l respectively. Neurokinin B (mumol/l) and calcitonin gene related peptide (1 mumol/l) did not inhibit binding. These binding sites show characteristics of the neurokinin 1 tachykinin receptor subtype. This provides further evidence that substance P may play a part in the vascular control of human synovium and may influence inflammatory processes in joints.

Cited by

References

  1. Arthritis Rheum. 1990 Jan;33(1):87-90 - PubMed
  2. Ann Rheum Dis. 1962 Dec;21:370-7 - PubMed
  3. Neuroscience. 1990;37(1):143-53 - PubMed
  4. Arthritis Rheum. 1990 Mar;33(3):412-7 - PubMed
  5. J Rheumatol. 1985 Jun;12(3):406-11 - PubMed
  6. Eur J Pharmacol. 1986 Nov 4;130(3):209-17 - PubMed
  7. Science. 1987 Feb 20;235(4791):893-5 - PubMed
  8. J Physiol. 1986 Oct;379:407-16 - PubMed
  9. Am Rev Respir Dis. 1987 Dec;136(6 Pt 2):S48-51 - PubMed
  10. Brain Res. 1988 Aug 23;458(2):319-24 - PubMed
  11. Can J Physiol Pharmacol. 1989 Feb;67(2):159-62 - PubMed
  12. J Biol Chem. 1989 Oct 25;264(30):17649-52 - PubMed
  13. Ann Rheum Dis. 1989 Nov;48(11):928-32 - PubMed
  14. Lancet. 1989 Nov 11;2(8672):1128-30 - PubMed
  15. Trends Pharmacol Sci. 1989 Jun;10(6):227-9 - PubMed
  16. Br J Pharmacol. 1989 Feb;96(2):418-24 - PubMed
  17. J Rheumatol. 1988 Dec;15(12):1807-10 - PubMed
  18. Neuroscience. 1988 Mar;24(3):739-68 - PubMed
  19. N Engl J Med. 1986 May 15;314(20):1323 - PubMed
  20. J Neurosci. 1986 Dec;6(12):3423-9 - PubMed
  21. Br Med J. 1966 Sep 24;2(5516):739-42 - PubMed
  22. Pharmacol Rev. 1983 Jun;35(2):85-141 - PubMed
  23. Science. 1984 Nov 2;226(4674):547-9 - PubMed
  24. Circ Res. 1983 Nov;53(5):557-73 - PubMed
  25. Br J Pharmacol. 1990 Mar;99(3):565-71 - PubMed

Substances

MeSH terms

Publication Types

LinkOut - more resources