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Zambonin CG, Aresta A. SPME-LC with UV detection to study delorazepam-serum albumin interactions. J Pharm Biomed Anal. 2002;29(5):895-900doi: 10.1016/s0731-7085(02)00217-0.
Zambonin, C. G., & Aresta, A. (2002). SPME-LC with UV detection to study delorazepam-serum albumin interactions. Journal of pharmaceutical and biomedical analysis, 29(5), 895-900. https://doi.org/10.1016/s0731-7085(02)00217-0
Zambonin, Carlo G, and Aresta, Antonella. "SPME-LC with UV detection to study delorazepam-serum albumin interactions." Journal of pharmaceutical and biomedical analysis vol. 29,5 (2002): 895-900. doi: https://doi.org/10.1016/s0731-7085(02)00217-0
Zambonin CG, Aresta A. SPME-LC with UV detection to study delorazepam-serum albumin interactions. J Pharm Biomed Anal. 2002 Jul 31;29(5):895-900. doi: 10.1016/s0731-7085(02)00217-0. PMID: 12093523.
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J Pharm Biomed Anal. 2002 Jul 31;29(5):895-900. doi: 10.1016/s0731-7085(02)00217-0.

SPME-LC with UV detection to study delorazepam-serum albumin interactions.

Journal of pharmaceutical and biomedical analysis

Carlo G Zambonin, Antonella Aresta

Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, 70126, Bari, Italy. [email protected]

PMID: 12093523 DOI: 10.1016/s0731-7085(02)00217-0

Abstract

Solid phase microextraction coupled to high performance liquid chromatography with UV detection (SPME/LC-UV) has been employed to study the binding of delorazepam to human serum albumin (HSA) and bovine serum albumin (BSA). The procedure could also be potentially extended to the measurement of partition coefficients between a wide variety of semi- or non-volatile compounds and matrices. The method is solvent free, simple, fast, and drawbacks of the conventional analytical techniques are avoided. Moreover, the matrix did not interfere with the measurement by binding to the fibre and the amount extracted by the fibre was negligibly small; thus it did not disturb the delorazepam-protein binding.

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