Display options
Cite
Kantelhardt SR, Wuhrer M, Dennis RD, et al. Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity. Biochem J. 2002;366:217-23doi: 10.1042/BJ20011678.
Kantelhardt, S. R., Wuhrer, M., Dennis, R. D., Doenhoff, M. J., Bickle, Q., & Geyer, R. (2002). Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity. The Biochemical journal, 366217-23. https://doi.org/10.1042/BJ20011678
Kantelhardt, Sven R, et al. "Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity." The Biochemical journal vol. 366 (2002): 217-23. doi: https://doi.org/10.1042/BJ20011678
Kantelhardt SR, Wuhrer M, Dennis RD, Doenhoff MJ, Bickle Q, Geyer R. Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity. Biochem J. 2002 Aug 15;366:217-23. doi: 10.1042/BJ20011678. PMID: 11996672; PMCID: PMC1222754.
Copy Download .nbib Format: NLM
Share it on

Biochem J. 2002 Aug 15;366:217-23. doi: 10.1042/BJ20011678.

Fuc(alpha1-->3)GalNAc-: the major antigenic motif of Schistosoma mansoni glycolipids implicated in infection sera and keyhole-limpet haemocyanin cross-reactivity.

The Biochemical journal

Sven R Kantelhardt, Manfred Wuhrer, Roger D Dennis, Michael J Doenhoff, Quentin Bickle, Rudolf Geyer

Affiliations

  1. Institute of Biochemistry, Faculty of Medicine, University of Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.

PMID: 11996672 PMCID: PMC1222754 DOI: 10.1042/BJ20011678

Abstract

The aim of the present study was the characterization of the dominant epitope present on Schistosoma mansoni glycolipids, which causes cross-reactivity of S. mansoni and S. haematobium infection sera with keyhole-limpet haemocyanin (KLH). To this end, the monoclonal antibody M2D3H was chosen for its similar behaviour in high-performance TLC immunostaining and inhibition-ELISA to infection sera. Individual, structurally defined oligosaccharides derived from S. mansoni egg glycolipids were tested for their binding to this monoclonal antibody by immunoaffinity chromatography. A terminal fucose residue linked in the (alpha1-->3) position to N-acetylgalactosamine was found to be the common structural determinant of the four oligosaccharides binding to M2D3H. The Fuc(alpha1-->3)GalNAc-motif also appeared to be the basis for the cross-reactivity with KLH, a phenomenon used in the serodiagnosis of S. mansoni, S. haematobium and S. japonicum infections.

References

  1. J Immunol. 1988 May 1;140(9):3273-9 - PubMed
  2. Glycobiology. 2000 Jan;10(1):89-101 - PubMed
  3. J Biol Chem. 1995 Jul 21;270(29):17114-23 - PubMed
  4. J Infect Dis. 2001 Apr 15;183(8):1238-47 - PubMed
  5. Vaccine. 1998 Aug;16(13):1290-8 - PubMed
  6. Nature. 1985 Jul 4-10;316(6023):74-6 - PubMed
  7. Eur J Biochem. 2002 Jan;269(2):481-93 - PubMed
  8. Trop Geogr Med. 1994;46(4 Spec No):255-8 - PubMed
  9. Infect Immun. 2001 Apr;69(4):2396-401 - PubMed
  10. Parasitology. 1999 Jan;118 ( Pt 1):83-9 - PubMed
  11. J Immunol. 1986 Dec 1;137(11):3601-7 - PubMed
  12. Am J Trop Med Hyg. 1989 Sep;41(3):338-44 - PubMed
  13. Parasite Immunol. 1986 Jan;8(1):95-107 - PubMed
  14. Nature. 1986 Oct 2-8;323(6087):443-5 - PubMed
  15. Anal Biochem. 1994 Aug 1;220(2):275-83 - PubMed
  16. Glycobiology. 2001 May;11(5):373-84 - PubMed
  17. Parasite Immunol. 1988 Mar;10(2):151-68 - PubMed
  18. Infect Immun. 1999 Nov;67(11):5946-50 - PubMed
  19. Glycobiology. 2000 Jun;10(6):601-9 - PubMed
  20. Glycobiology. 2000 Feb;10(2):223-9 - PubMed
  21. Trans R Soc Trop Med Hyg. 1992 Jan-Feb;86(1):53-6 - PubMed
  22. Mol Biochem Parasitol. 1999 Oct 15;103(2):155-69 - PubMed
  23. Parasitol Today. 1998 Mar;14(3):105-9 - PubMed
  24. Mol Biochem Parasitol. 2000 Oct;110(2):237-46 - PubMed
  25. J Exp Med. 1987 Mar 1;165(3):865-78 - PubMed
  26. Trans R Soc Trop Med Hyg. 1994 Mar-Apr;88(2):249-51 - PubMed
  27. Glycobiology. 1997 Jul;7(5):653-61 - PubMed

Substances

MeSH terms

Publication Types