Display options
Cite
Erickson DL, Endersby R, Kirkham A, et al. Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis. Infect Immun. 2002;70(4):1783-90doi: 10.1128/IAI.70.4.1783-1790.2002.
Erickson, D. L., Endersby, R., Kirkham, A., Stuber, K., Vollman, D. D., Rabin, H. R., Mitchell, I., & Storey, D. G. (2002). Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis. Infection and immunity, 70(4), 1783-90. https://doi.org/10.1128/IAI.70.4.1783-1790.2002
Erickson, David L, et al. "Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis." Infection and immunity vol. 70,4 (2002): 1783-90. doi: https://doi.org/10.1128/IAI.70.4.1783-1790.2002
Erickson DL, Endersby R, Kirkham A, Stuber K, Vollman DD, Rabin HR, Mitchell I, Storey DG. Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis. Infect Immun. 2002 Apr;70(4):1783-90. doi: 10.1128/IAI.70.4.1783-1790.2002. PMID: 11895939; PMCID: PMC127834.
Copy Download .nbib Format: NLM
Share it on

Infect Immun. 2002 Apr;70(4):1783-90. doi: 10.1128/IAI.70.4.1783-1790.2002.

Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis.

Infection and immunity

David L Erickson, Ryan Endersby, Amanda Kirkham, Kent Stuber, Dolina D Vollman, Harvey R Rabin, Ian Mitchell, Douglas G Storey

Affiliations

  1. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4.

PMID: 11895939 PMCID: PMC127834 DOI: 10.1128/IAI.70.4.1783-1790.2002

Abstract

Individuals with cystic fibrosis (CF) are commonly colonized with Pseudomonas aeruginosa. The chronic infections caused by P. aeruginosa are punctuated by acute exacerbations of the lung disease, which lead to significant morbidity and mortality. As regulators of virulence determinants, P. aeruginosa quorum-sensing systems may be active in the chronic lung infections associated with CF. We have examined the levels of autoinducer molecules and transcript accumulation from the bacterial populations found in the lungs of patients with CF. We detected biologically active levels of N-(3-oxododecanoyl)-L-homoserine (3-oxo-C12-HSL) and N-butyryl-L-homoserine lactone (C4-HSL) in sputum from CF patients. Interestingly, it appears that C4-HSL is less frequently detected than 3-oxo-C12-HSL in the lungs of patients with CF. We also examined the transcription of the autoinducer synthase gene lasI and showed that it is frequently expressed in the lungs of patients with CF. We observed a significant correlation between the expression of lasI and four target genes of the Las quorum-sensing system. Taken together, our results indicate that quorum-sensing systems are active and may control virulence factor expression in the lungs of patients with CF.

References

  1. J Bacteriol. 1999 Sep;181(17):5489-97 - PubMed
  2. FEMS Microbiol Lett. 2000 Mar 15;184(2):273-8 - PubMed
  3. J Bacteriol. 1980 Jun;142(3):836-42 - PubMed
  4. J Bacteriol. 1988 Oct;170(10):4477-83 - PubMed
  5. J Clin Microbiol. 1987 Oct;25(10):1868-74 - PubMed
  6. Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3526-31 - PubMed
  7. Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):197-201 - PubMed
  8. J Bacteriol. 2001 Sep;183(18):5213-22 - PubMed
  9. Science. 1993 May 21;260(5111):1127-30 - PubMed
  10. Infect Immun. 1992 Nov;60(11):4687-94 - PubMed
  11. J Bacteriol. 1999 Feb;181(3):748-56 - PubMed
  12. Infect Immun. 1997 Oct;65(10):4061-7 - PubMed
  13. Science. 1999 Nov 19;286(5444):1561-5 - PubMed
  14. Annu Rev Genet. 1995;29:777-807 - PubMed
  15. Annu Rev Microbiol. 1996;50:727-51 - PubMed
  16. Acta Paediatr Scand. 1984 Nov;73(6):772-7 - PubMed
  17. Environ Microbiol. 2000 Oct;2(5):530-41 - PubMed
  18. Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1639-44 - PubMed
  19. J Bacteriol. 1989 Oct;171(10):5304-13 - PubMed
  20. Antimicrob Agents Chemother. 2000 Mar;44(3):710-2 - PubMed
  21. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11229-34 - PubMed
  22. J Bacteriol. 1997 Sep;179(18):5756-67 - PubMed
  23. Infect Immun. 1998 Jan;66(1):36-42 - PubMed
  24. Appl Environ Microbiol. 2001 Apr;67(4):1865-73 - PubMed
  25. J Bacteriol. 1999 Apr;181(7):2175-84 - PubMed
  26. J Infect Dis. 1978 Jul;138(1):49-8 - PubMed
  27. J Clin Microbiol. 1982 Jun;15(6):1054-8 - PubMed
  28. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2848-9 - PubMed
  29. J Bacteriol. 1991 May;173(9):3000-9 - PubMed
  30. Infect Immun. 1999 Oct;67(10):5076-82 - PubMed
  31. J Mol Biol. 2001 Jun 8;309(3):631-40 - PubMed
  32. Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1490-4 - PubMed
  33. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6036-41 - PubMed
  34. J Bacteriol. 1996 Jan;178(2):435-40 - PubMed
  35. Microbiology. 2000 Oct;146 ( Pt 10):2481-93 - PubMed
  36. Microbiology. 2001 May;147(Pt 5):1105-13 - PubMed
  37. Nature. 2000 Oct 12;407(6805):762-4 - PubMed
  38. Mol Microbiol. 1997 Jun;24(5):895-904 - PubMed
  39. J Bacteriol. 1998 Oct;180(20):5398-405 - PubMed
  40. Microbes Infect. 2000 Nov;2(14):1721-31 - PubMed
  41. Nucleic Acids Res. 1989 Dec 25;17(24):10509 - PubMed
  42. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6424-8 - PubMed
  43. J Bacteriol. 1999 Feb;181(4):1203-10 - PubMed
  44. Science. 2000 May 19;288(5469):1251-4 - PubMed
  45. N Engl J Med. 1995 Mar 23;332(12):819-20; author reply 820-1 - PubMed
  46. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13904-9 - PubMed
  47. Infect Immun. 1998 Jun;66(6):2521-8 - PubMed
  48. Bioorg Med Chem Lett. 1999 Dec 20;9(24):3447-52 - PubMed

Substances

MeSH terms

Publication Types