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Din L, Li L, Tao P, et al. Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;767(1):75-81doi: 10.1016/s0378-4347(01)00546-1.
Din, L., Li, L., Tao, P., Yang, J., & Zhang, Z. (2002). Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 767(1), 75-81. https://doi.org/10.1016/s0378-4347(01)00546-1
Din, Li, et al. "Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry." Journal of chromatography. B, Analytical technologies in the biomedical and life sciences vol. 767,1 (2002): 75-81. doi: https://doi.org/10.1016/s0378-4347(01)00546-1
Din L, Li L, Tao P, Yang J, Zhang Z. Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Feb 05;767(1):75-81. doi: 10.1016/s0378-4347(01)00546-1. PMID: 11863298.
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J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Feb 05;767(1):75-81. doi: 10.1016/s0378-4347(01)00546-1.

Quantitation of tamsulosin in human plasma by liquid chromatography-electrospray ionization mass spectrometry.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Li Din, Limin Li, Ping Tao, Jin Yang, Zhengxing Zhang

Affiliations

  1. Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing. [email protected]

PMID: 11863298 DOI: 10.1016/s0378-4347(01)00546-1

Abstract

A highly sensitive method for quantitation of tamsulosin in human plasma using 1-(2,6-dimethyl-3-hydroxylphenoxy)-2-(3,4-methoxyphenylethylamino)-propane hydrochloride as the internal standard (I.S.) was established using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). After alkalization with saturated sodium bicarbonate, plasma were extracted by ethyl acetate and separated by HPLC on a C18 reversed-phase column using a mobile phase of methanol-water-acetic acid-triethylamine (620:380:1.5:1.5, v/v). Analytes were quantitated using positive electrospray ionization in a quadrupole spectrometer. LC-ESI-MS was performed in the selected ion monitoring (SIM) mode using target ions at m/z 228 for tamsulosin and m/z 222 for the I.S. Calibration curves, which were linear over the range 0.2-30 ng/ml, were analyzed contemporaneously with each batch of samples, along with low (0.5 ng/ml), medium (3 ng/ml) and high (30 ng/ml) quality control samples. The intra- and inter-assay variability ranged from 2.14 to 8.87% for the low, medium and high quality control samples. The extraction recovery of tamsulosin from plasma was in the range of 84.2-94.5%. The method has been used successfully to study tamsulosin pharmacokinetics in adult humans.

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