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van Remoortere A, van Dam GJ, Hokke CH, et al. Profiles of immunoglobulin M (IgM) and IgG antibodies against defined carbohydrate epitopes in sera of Schistosoma-infected individuals determined by surface plasmon resonance. Infect Immun. 2001;69(4):2396-401doi: 10.1128/IAI.69.4.2396-2401.2001.
van Remoortere, A., van Dam, G. J., Hokke, C. H., van den Eijnden, D. H., van Die, I., & Deelder, A. M. (2001). Profiles of immunoglobulin M (IgM) and IgG antibodies against defined carbohydrate epitopes in sera of Schistosoma-infected individuals determined by surface plasmon resonance. Infection and immunity, 69(4), 2396-401. https://doi.org/10.1128/IAI.69.4.2396-2401.2001
van Remoortere, A, et al. "Profiles of immunoglobulin M (IgM) and IgG antibodies against defined carbohydrate epitopes in sera of Schistosoma-infected individuals determined by surface plasmon resonance." Infection and immunity vol. 69,4 (2001): 2396-401. doi: https://doi.org/10.1128/IAI.69.4.2396-2401.2001
van Remoortere A, van Dam GJ, Hokke CH, van den Eijnden DH, van Die I, Deelder AM. Profiles of immunoglobulin M (IgM) and IgG antibodies against defined carbohydrate epitopes in sera of Schistosoma-infected individuals determined by surface plasmon resonance. Infect Immun. 2001 Apr;69(4):2396-401. doi: 10.1128/IAI.69.4.2396-2401.2001. PMID: 11254599; PMCID: PMC98171.
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Infect Immun. 2001 Apr;69(4):2396-401. doi: 10.1128/IAI.69.4.2396-2401.2001.

Profiles of immunoglobulin M (IgM) and IgG antibodies against defined carbohydrate epitopes in sera of Schistosoma-infected individuals determined by surface plasmon resonance.

Infection and immunity

A van Remoortere, G J van Dam, C H Hokke, D H van den Eijnden, I van Die, A M Deelder

Affiliations

  1. Department of Parasitology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.

PMID: 11254599 PMCID: PMC98171 DOI: 10.1128/IAI.69.4.2396-2401.2001

Abstract

We report here that sera of children and adults infected with Schistosoma mansoni, S. haematobium, or S. japonicum contain antibodies against GalNAcbeta1-4(Fucalpha1-2Fucalpha1-3)GlcNAc (LDN-DF) and to a lesser extent to Galbeta1-4(Fucalpha1-3)GlcNAc (Lewis(x)) and GalNAcbeta1-4GlcNAc (LDN). Surface plasmon resonance (SPR) spectroscopy was used to monitor the presence of serum antibodies to neoglycoconjugates containing these carbohydrate epitopes and to define the immunoglobulin M (IgM) and IgG subclass distribution of the antibodies. The serum levels of antibodies to LDN-DF are high related to LDN and Lewis(x) for all examined groups of Schistosoma-infected individuals. A higher antibody response to the LDN-DF epitope was found in sera of infected children than in sera of infected adults regardless of the schistosome species. With respect to the subclasses, we found surprisingly that individuals infected with S. japonicum have predominantly IgG antibodies, while individuals infected with S. mansoni mainly show an IgM response; high levels of both isotypes were measured in sera of individuals infected with S. haematobium. These data provide new insights in the human humoral immune response to schistosome-derived glycans.

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