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Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay.

Toxicology in vitro : an international journal published in association with BIBRA

Ritter D, Knebel J.
PMID: 19595757
Toxicol In Vitro. 2009 Dec;23(8):1570-5. doi: 10.1016/j.tiv.2009.07.007. Epub 2009 Jul 10.

Higher throughput methods, high content analysis and automated screening methods are of highest demand in drug development today. In toxicology, these strategies are becoming increasingly important, as well. Therefore, an integrated higher throughput method for the comet assay is...

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Ritter D, Knebel J. Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay. Toxicol In Vitro. 2009;23(8):1570-5doi: 10.1016/j.tiv.2009.07.007.
Ritter, D., & Knebel, J. (2009). Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay. Toxicology in vitro : an international journal published in association with BIBRA, 23(8), 1570-5. https://doi.org/10.1016/j.tiv.2009.07.007
Ritter, Detlef, and Knebel, Jan. "Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay." Toxicology in vitro : an international journal published in association with BIBRA vol. 23,8 (2009): 1570-5. doi: https://doi.org/10.1016/j.tiv.2009.07.007
Ritter D, Knebel J. Genotoxicity testing in vitro - development of a higher throughput analysis method based on the comet assay. Toxicol In Vitro. 2009 Dec;23(8):1570-5. doi: 10.1016/j.tiv.2009.07.007. Epub 2009 Jul 10. PMID: 19595757.
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Comments on 'A hierarchical zero-inflated log-normal model for skewed responses'.

Statistical methods in medical research

Liu L, Ma JZ.
PMID: 21849354
Stat Methods Med Res. 2011 Aug;20(4):445. doi: 10.1177/0962280209357986.

No abstract available.

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Liu L, Ma JZ. Comments on 'A hierarchical zero-inflated log-normal model for skewed responses'. Stat Methods Med Res. 2011;20(4):445doi: 10.1177/0962280209357986.
Liu, L., & Ma, J. Z. (2011). Comments on 'A hierarchical zero-inflated log-normal model for skewed responses'. Statistical methods in medical research, 20(4), 445. https://doi.org/10.1177/0962280209357986
Liu, Lei, and Ma, Jennie Z. "Comments on 'A hierarchical zero-inflated log-normal model for skewed responses'." Statistical methods in medical research vol. 20,4 (2011): 445. doi: https://doi.org/10.1177/0962280209357986
Liu L, Ma JZ. Comments on 'A hierarchical zero-inflated log-normal model for skewed responses'. Stat Methods Med Res. 2011 Aug;20(4):445. doi: 10.1177/0962280209357986. PMID: 21849354.
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Additional tracts on the comet of 1577.

Isis; an international review devoted to the history of science and its cultural influences

HELLMAN CD.
PMID: 18879008
Isis. 1948 Aug;39(117):172-4. doi: 10.1086/348951.

No abstract available.

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HELLMAN CD. Additional tracts on the comet of 1577. Isis. 1948;39(117):172-4doi: 10.1086/348951.
HELLMAN, C. D. (1948). Additional tracts on the comet of 1577. Isis; an international review devoted to the history of science and its cultural influences, 39(117), 172-4. https://doi.org/10.1086/348951
HELLMAN, C D. "Additional tracts on the comet of 1577." Isis; an international review devoted to the history of science and its cultural influences vol. 39,117 (1948): 172-4. doi: https://doi.org/10.1086/348951
HELLMAN CD. Additional tracts on the comet of 1577. Isis. 1948 Aug;39(117):172-4. doi: 10.1086/348951. PMID: 18879008.
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CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells.

Journal of visualized experiments : JoVE

Ge J, Prasongtanakij S, Wood DK, Weingeist DM, Fessler J, Navasummrit P, Ruchirawat M, Engelward BP.
PMID: 25350601
J Vis Exp. 2014 Oct 18;(92):e50607. doi: 10.3791/50607.

DNA damaging agents can promote aging, disease and cancer and they are ubiquitous in the environment and produced within human cells as normal cellular metabolites. Ironically, at high doses DNA damaging agents are also used to treat cancer. The...

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Ge J, Prasongtanakij S, Wood DK, et al. CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. J Vis Exp. 2014;(92):e50607doi: 10.3791/50607.
Ge, J., Prasongtanakij, S., Wood, D. K., Weingeist, D. M., Fessler, J., Navasummrit, P., Ruchirawat, M., & Engelward, B. P. (2014). CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of visualized experiments : JoVE, (92), e50607. https://doi.org/10.3791/50607
Ge, Jing, et al. "CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells." Journal of visualized experiments : JoVE vol. ,92 (2014): e50607. doi: https://doi.org/10.3791/50607
Ge J, Prasongtanakij S, Wood DK, Weingeist DM, Fessler J, Navasummrit P, Ruchirawat M, Engelward BP. CometChip: a high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. J Vis Exp. 2014 Oct 18;(92):e50607. doi: 10.3791/50607. PMID: 25350601; PMCID: PMC4407627.
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Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay.

Mutagenesis

Huk A, Collins AR, El Yamani N, Porredon C, Azqueta A, de Lapuente J, Dusinska M.
PMID: 25527731
Mutagenesis. 2015 Jan;30(1):85-8. doi: 10.1093/mutage/geu077.

The comet assay is widely used to test the genotoxicity of engineered nanomaterials (ENMs) but outcomes may vary when results from different laboratories, or even within one laboratory, are compared. We address some basic methodological considerations, such as the...

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Huk A, Collins AR, El Yamani N, et al. Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis. 2015;30(1):85-8doi: 10.1093/mutage/geu077.
Huk, A., Collins, A. R., El Yamani, N., Porredon, C., Azqueta, A., de Lapuente, J., & Dusinska, M. (2015). Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis, 30(1), 85-8. https://doi.org/10.1093/mutage/geu077
Huk, Anna, et al. "Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay." Mutagenesis vol. 30,1 (2015): 85-8. doi: https://doi.org/10.1093/mutage/geu077
Huk A, Collins AR, El Yamani N, Porredon C, Azqueta A, de Lapuente J, Dusinska M. Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis. 2015 Jan;30(1):85-8. doi: 10.1093/mutage/geu077. PMID: 25527731.
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Special issue on the 20th anniversary of the comet assay.

Mutation research

Rojas E.
PMID: 18773969
Mutat Res. 2009 Jan-Feb;681(1):1-2. doi: 10.1016/j.mrrev.2008.08.001. Epub 2008 Aug 19.

No abstract available.

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Rojas E. Special issue on the 20th anniversary of the comet assay. Mutat Res. 2008;681(1):1-2doi: 10.1016/j.mrrev.2008.08.001.
Rojas, E. (2009). Special issue on the 20th anniversary of the comet assay. Mutation research, 681(1), 1-2. https://doi.org/10.1016/j.mrrev.2008.08.001
Rojas, Emilio. "Special issue on the 20th anniversary of the comet assay." Mutation research vol. 681,1 (2009): 1-2. doi: https://doi.org/10.1016/j.mrrev.2008.08.001
Rojas E. Special issue on the 20th anniversary of the comet assay. Mutat Res. 2009 Jan-Feb;681(1):1-2. doi: 10.1016/j.mrrev.2008.08.001. Epub 2008 Aug 19. PMID: 18773969.
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[Modification of conventional single cell gel electrophoresis.].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

Luo MZ, Qi H, Qu Y.
PMID: 18226373
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Nov;25(11):674-5.

No abstract available.

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Luo MZ, Qi H, Qu Y. [Modification of conventional single cell gel electrophoresis.]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007;25(11):674-5
Luo, M. Z., Qi, H., & Qu, Y. (2007). [Modification of conventional single cell gel electrophoresis.]. Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, 25(11), 674-5.
Luo, Ming-Zhi, et al. "[Modification of conventional single cell gel electrophoresis.]." Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases vol. 25,11 (2007): 674-5.
Luo MZ, Qi H, Qu Y. [Modification of conventional single cell gel electrophoresis.]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Nov;25(11):674-5. Chinese. PMID: 18226373.
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The comet assay: a heavenly method!.

Mutagenesis

Collins AR.
PMID: 25527721
Mutagenesis. 2015 Jan;30(1):1-4. doi: 10.1093/mutage/geu079.

The contributions to this special issue of Mutagenesis have been selected to cover the main research areas served by the comet assay, namely genotoxicology, environmental toxicology, human biomonitoring and fundamental investigations into mechanisms of DNA damage and repair. Innovative...

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Collins AR. The comet assay: a heavenly method!. Mutagenesis. 2015;30(1):1-4doi: 10.1093/mutage/geu079.
Collins, A. R. (2015). The comet assay: a heavenly method!. Mutagenesis, 30(1), 1-4. https://doi.org/10.1093/mutage/geu079
Collins, Andrew R. "The comet assay: a heavenly method!." Mutagenesis vol. 30,1 (2015): 1-4. doi: https://doi.org/10.1093/mutage/geu079
Collins AR. The comet assay: a heavenly method!. Mutagenesis. 2015 Jan;30(1):1-4. doi: 10.1093/mutage/geu079. PMID: 25527721.
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The Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility.

Scientific reports

Braafladt S, Reipa V, Atha DH.
PMID: 27581626
Sci Rep. 2016 Sep 01;6:32162. doi: 10.1038/srep32162.

Sources of variability in the comet assay include variations in the protocol used to process the cells, the microscope imaging system and the software used in the computerized analysis of the images. Here we focus on the effect of...

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Braafladt S, Reipa V, Atha DH. The Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility. Sci Rep. 2016;6:32162doi: 10.1038/srep32162.
Braafladt, S., Reipa, V., & Atha, D. H. (2016). The Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility. Scientific reports, 632162. https://doi.org/10.1038/srep32162
Braafladt, Signe, et al. "The Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility." Scientific reports vol. 6 (2016): 32162. doi: https://doi.org/10.1038/srep32162
Braafladt S, Reipa V, Atha DH. The Comet Assay: Automated Imaging Methods for Improved Analysis and Reproducibility. Sci Rep. 2016 Sep 01;6:32162. doi: 10.1038/srep32162. PMID: 27581626; PMCID: PMC5007470.
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Single nucleus versus single-cell gel electrophoresis: kinetics of DNA track formation.

Electrophoresis

Afanasieva K, Chopei M, Sivolob A.
PMID: 25631953
Electrophoresis. 2015 Apr;36(7):973-7. doi: 10.1002/elps.201400566. Epub 2015 Mar 24.

Single-cell gel electrophoresis, or the comet assay, is usually performed with nucleoids prepared after a lysis of either whole cells (more often) or isolated cell nuclei (rarely). Electrophoretic properties of the second type of nucleoids have never been investigated...

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Afanasieva K, Chopei M, Sivolob A. Single nucleus versus single-cell gel electrophoresis: kinetics of DNA track formation. Electrophoresis. 2015;36(7):973-7doi: 10.1002/elps.201400566.
Afanasieva, K., Chopei, M., & Sivolob, A. (2015). Single nucleus versus single-cell gel electrophoresis: kinetics of DNA track formation. Electrophoresis, 36(7), 973-7. https://doi.org/10.1002/elps.201400566
Afanasieva, Katerina, et al. "Single nucleus versus single-cell gel electrophoresis: kinetics of DNA track formation." Electrophoresis vol. 36,7 (2015): 973-7. doi: https://doi.org/10.1002/elps.201400566
Afanasieva K, Chopei M, Sivolob A. Single nucleus versus single-cell gel electrophoresis: kinetics of DNA track formation. Electrophoresis. 2015 Apr;36(7):973-7. doi: 10.1002/elps.201400566. Epub 2015 Mar 24. PMID: 25631953.
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Showing 1 to 10 of 10 entries