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An improved workflow for quantitative N‐terminal charge‐based fractional diagonal chromatography (ChaFRADIC) to study proteolytic events in Arabidopsis thaliana

science campus

[No authors listed]
GSID: TwIp04NPb3oJ
AS Venne, FA Solari, F Faden, T Paretti… - …, 2015 - Wiley Online Library

We applied an extended charge‐based fractional diagonal chromatography (ChaFRADIC) workflow to analyze the N‐terminal proteome of Arabidopsis thaliana seedlings. Using iTRAQ protein labeling and a multi‐enzyme digestion approach including trypsin, GluC, and …

The effects of oral hydrolytic enzymes and flavonoids on inflammatory markers and coagulation after marathon running: study protocol for a randomized, double-blind, placebo-controlled trial.

BMC sports science, medicine & rehabilitation

Grabs V, Nieman DC, Haller B, Halle M, Scherr J.
PMID: 24559067
BMC Sports Sci Med Rehabil. 2014 Feb 22;6(1):8. doi: 10.1186/2052-1847-6-8.

BACKGROUND: Regular moderate intensity physical activity positively influences the immune system with a lower incidence of upper respiratory tract infections (URTI) and lower levels of pro-inflammatory markers. However, marathon running due to its strenuous and prolonged nature results in...

Pancreas procurement and preservation for islet transplantation: personal considerations.

Journal of transplantation

Noguchi H.
PMID: 21918716
J Transplant. 2011;2011:783168. doi: 10.1155/2011/783168. Epub 2011 Sep 12.

Pancreatic islet transplantation is a promising option for the treatment of type 1 diabetic patients. After the successful demonstration of the Edmonton protocol, islet transplantation has advanced significantly on several fronts, including improved pancreas procurement and preservation systems. Since...

Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization.

Cytotechnology

Jin M, Wu A, Dorzhin S, Yue Q, Ma Y, Liu D.
PMID: 22438181
Cytotechnology. 2012 Aug;64(4):379-89. doi: 10.1007/s10616-011-9408-z. Epub 2012 Mar 22.

Although isolation and characterization of embryonic stem cells have been successful in cattle, maintenance of bovine embryonic stem cells in culture remains difficult. In this study, we compared different methods of cell passaging, feeder cell layers and medium conditions...

The effect of culture on the neuronotrophic activity of avian smooth muscle.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Abrahamson IK, Murdoch SY, Rush RA.
PMID: 24874037
Int J Dev Neurosci. 1984;2(3):229-39. doi: 10.1016/0736-5748(84)90017-0.

The expansor secundariorum is a smooth muscle of the chicken wing which receives a dense noradrenergic innervation and contains high concentrations of trophic activity for sympathetic neurons. Expansor cells were dissociated in trypsin and grown to confluency in standard...

Real-time Crystal Growth Visualization and Quantification by Energy-Resolved Neutron Imaging.

Scientific reports

Tremsin AS, Perrodin D, Losko AS, Vogel SC, Bourke MAM, Bizarri GA, Bourret ED.
PMID: 28425461
Sci Rep. 2017 Apr 20;7:46275. doi: 10.1038/srep46275.

Energy-resolved neutron imaging is investigated as a real-time diagnostic tool for visualization and in-situ measurements of "blind" processes. This technique is demonstrated for the Bridgman-type crystal growth enabling remote and direct measurements of growth parameters crucial for process optimization....

Comparative evaluation of tannic acid inhibiting α-glucosidase and trypsin.

Food research international (Ottawa, Ont.)

Xiao H, Liu B, Mo H, Liang G.
PMID: 28455043
Food Res Int. 2015 Oct;76:605-610. doi: 10.1016/j.foodres.2015.07.029. Epub 2015 Jul 22.

In this work, the inhibitory effects of tannic acid on the α-glucosidase and trypsin were systematically evaluated by comparing with the clinical diabetes healer acarbose and the soybean-derived trypsin inhibitor using fluorescence spectroscopy and enzymatic kinetics methods. We showed...

Investigation of dissimilar metal welds by energy-resolved neutron imaging.

Journal of applied crystallography

Tremsin AS, Ganguly S, Meco SM, Pardal GR, Shinohara T, Feller WB.
PMID: 27504075
J Appl Crystallogr. 2016 Jun 09;49:1130-1140. doi: 10.1107/S1600576716006725. eCollection 2016 Aug 01.

A nondestructive study of the internal structure and compositional gradient of dissimilar metal-alloy welds through energy-resolved neutron imaging is described in this paper. The ability of neutrons to penetrate thick metal objects (up to several cm) provides a unique...

Introducing Mammalian Cell Culture and Cell Viability Techniques in the Undergraduate Biology Laboratory.

Journal of microbiology & biology education

Bowey-Dellinger K, Dixon L, Ackerman K, Vigueira C, Suh YK, Lyda T, Sapp K, Grider M, Crater D, Russell T, Elias M, Coffield VM, Segarra VA.
PMID: 28861134
J Microbiol Biol Educ. 2017 Aug 11;18(2). doi: 10.1128/jmbe.v18i2.1264. eCollection 2017.

Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more...

Mass spectrometry-based analyses showing the effects of secretor and blood group status on salivary N-glycosylation.

Clinical proteomics

Albertolle ME, Hassis ME, Ng CJ, Cuison S, Williams K, Prakobphol A, Dykstra AB, Hall SC, Niles RK, Ewa Witkowska H, Fisher SJ.
PMID: 26719750
Clin Proteomics. 2015 Dec 30;12:29. doi: 10.1186/s12014-015-9100-y. eCollection 2015.

BACKGROUND: The carbohydrate portions of salivary glycoproteins play important roles, including mediating bacterial and leukocyte adhesion. Salivary glycosylation is complex. Many of its glycoproteins present ABO and Lewis blood group determinants. An individual's genetic complement and secretor status govern...

Coarse-Graining the Accessible Surface and the Electrostatics of Proteins for Protein-Protein Interactions.

Journal of chemical theory and computation

Pizzitutti F, Marchi M, Borgis D.
PMID: 26627629
J Chem Theory Comput. 2007 Sep;3(5):1867-76. doi: 10.1021/ct700121n.

This study is concerned with the development and test of a coarse-grained representation specifically constructed for proteins and peptides, where each amino acid of the sequence is represented by a charged dipolar sphere. The model was parametrized from the...

Necrotic Response to Low Pathogenic H9N2 Influenza Virus in Chicken Hepatoma Cells.

Jundishapur journal of microbiology

Mosavi SZ, Shahsavandi S, Ebrahimi MM, Hatami AR, Sadeghi K, Shahivandi H.
PMID: 25789133
Jundishapur J Microbiol. 2015 Jan 25;8(1):e13770. doi: 10.5812/jjm.13770. eCollection 2015 Jan.

BACKGROUND: Limited knowledge about the molecular mechanism of avian influenza H9N2 virus pathogenicity in birds as well as human hosts has limited the development of effective control against the disease. To overcome this issue detailed understanding of the infectious...

Showing 1 to 12 of 2903 entries