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Showing 1 to 12 of 80 entries
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A proposed mechanism for IS607-family serine transposases.

Mobile DNA

Boocock MR, Rice PA.
PMID: 24195768
Mob DNA. 2013 Nov 06;4(1):24. doi: 10.1186/1759-8753-4-24.

BACKGROUND: The transposases encoded by the IS607 family of mobile elements are unusual serine recombinases with an inverted domain order and minimal specificity for target DNA.RESULTS: Structural genomics groups have determined three crystal structures of the catalytic domains of...

Recommended Method for Chromosome Exploitation: RMCE-based Cassette-exchange Systems in Animal Cell Biotechnology.

Cytotechnology

Oumard A, Qiao J, Jostock T, Li J, Bode J.
PMID: 19003073
Cytotechnology. 2006 Mar;50(1):93-108. doi: 10.1007/s10616-006-6550-0. Epub 2006 Jun 14.

The availability of site-specific recombinases has revolutionized the rational construction of cell lines with predictable properties. Early efforts were directed to providing pre-characterized genomic loci with a single recombinase target site that served as an address for the integration...

Function, replication and structure of the mammalian telomere.

Cytotechnology

Broccoli D.
PMID: 19003238
Cytotechnology. 2004 Jun;45(1):3-12. doi: 10.1007/s10616-004-5120-6.

Telomeres are specialized structures at the ends of linear chromosomes that were originally defined functionally based on observations first by Muller (1938) and subsequently by McClintock (1941) that naturally occurring chromosome ends do not behave as double-stranded DNA breaks,...

Plant DNA recombinases: a long way to go.

Journal of nucleic acids

Chittela RK, Sainis JK.
PMID: 20798837
J Nucleic Acids. 2010;2010. doi: 10.4061/2010/646109. Epub 2009 Dec 13.

DNA homologous recombination is fundamental process by which two homologous DNA molecules exchange the genetic information for the generation of genetic diversity and maintain the genomic integrity. DNA recombinases, a special group of proteins bind to single stranded DNA...

Two Subpopulations of Noradrenergic Neurons in the Locus Coeruleus Complex Distinguished by Expression of the Dorsal Neural Tube Marker .

Frontiers in neuroanatomy

Plummer NW, Scappini EL, Smith KG, Tucker CJ, Jensen P.
PMID: 28775681
Front Neuroanat. 2017 Jul 20;11:60. doi: 10.3389/fnana.2017.00060. eCollection 2017.

Central noradrenergic neurons, collectively defined by synthesis of the neurotransmitter norepinephrine, are a diverse collection of cells in the hindbrain, differing in their anatomy, physiological and behavioral functions, and susceptibility to disease and environmental insult. To investigate the developmental...

Loss of H3K36 Methyltransferase SETD2 Impairs V(D)J Recombination during Lymphoid Development.

iScience

Chu SH, Chabon JR, Matovina CN, Minehart JC, Chen BR, Zhang J, Kumar V, Xiong Y, Callen E, Hung PJ, Feng Z, Koche RP, Liu XS, Chaudhuri J, Nussenzweig A, Sleckman BP, Armstrong SA.
PMID: 32169821
iScience. 2020 Mar 27;23(3):100941. doi: 10.1016/j.isci.2020.100941. Epub 2020 Feb 27.

Repair of DNA double-stranded breaks (DSBs) during lymphocyte development is essential for V(D)J recombination and forms the basis of immunoglobulin variable region diversity. Understanding of this process in lymphogenesis has historically been centered on the study of RAG1/2 recombinases...

Archaeal tyrosine recombinases.

FEMS microbiology reviews

Badel C, Da Cunha V, Oberto J.
PMID: 33524101
FEMS Microbiol Rev. 2021 Aug 17;45(4). doi: 10.1093/femsre/fuab004.

The integration of mobile genetic elements into their host chromosome influences the immediate fate of cellular organisms and gradually shapes their evolution. Site-specific recombinases catalyzing this integration have been extensively characterized both in bacteria and eukarya. More recently, a...

Salmonella Genomic Island 1 requires a self-encoded small RNA for mobilization.

Molecular microbiology

Nagy I, Szabó M, Hegyi A, Kiss J.
PMID: 34784078
Mol Microbiol. 2021 Nov 16; doi: 10.1111/mmi.14846. Epub 2021 Nov 16.

The SGI1-family elements that are specifically mobilized by the IncA- and IncC-family plasmids are important vehicles of antibiotic resistance among enteric bacteria. Although SGI1 exploits many plasmid-derived conjugation and regulatory functions, the basic mobilization module of the island is...

Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination.

Nucleic acids research

Xu J, Zhao L, Peng S, Chu H, Liang R, Tian M, Connell PP, Li G, Chen C, Wang HW.
PMID: 34871438
Nucleic Acids Res. 2021 Dec 06; doi: 10.1093/nar/gkab1141. Epub 2021 Dec 06.

Homologous recombination (HR) is a primary DNA double-strand breaks (DSBs) repair mechanism. The recombinases Rad51 and Dmc1 are highly conserved in the RecA family; Rad51 is mainly responsible for DNA repair in somatic cells during mitosis while Dmc1 only...

Progress in the molecular biology of ewing tumors.

Sarcoma

Kovar H.
PMID: 18521227
Sarcoma. 1998;2(1):3-17. doi: 10.1080/13577149878109.

Purpose/results/discussion. Rearrangement of the EWS gene with an ETS oncogene by chromosomal translocation is a hallmark of the Ewing family of tumors (EFT). Detectability, incidence, tumor specificity and variability of this aberration have been matters of intense investigation in...

Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR).

STAR protocols

Rincon Fernandez Pacheco D, Sabet S, Breunig JJ.
PMID: 33377093
STAR Protoc. 2020 Dec 09;1(3):100199. doi: 10.1016/j.xpro.2020.100199. eCollection 2020 Dec 18.

This protocol focuses on the cloning and stable integration of sequences of interest by the use of a mosaic analysis with dual recombinases (MADR) plasmid that includes fusion proteins or independent proteins under the control of 2A peptide or...

Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination.

Nucleic acids research

Xu J, Zhao L, Peng S, Chu H, Liang R, Tian M, Connell PP, Li G, Chen C, Wang HW.
PMID: 34871438
Nucleic Acids Res. 2021 Dec 16;49(22):13135-13149. doi: 10.1093/nar/gkab1141.

Homologous recombination (HR) is a primary DNA double-strand breaks (DSBs) repair mechanism. The recombinases Rad51 and Dmc1 are highly conserved in the RecA family; Rad51 is mainly responsible for DNA repair in somatic cells during mitosis while Dmc1 only...

Showing 1 to 12 of 80 entries