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Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System.

Methods in molecular biology (Clifton, N.J.)

Janmohamed A, Thaunsukon P, Shephard EA, Phillips IR.
PMID: 27699678
Methods Mol Biol. 2006;320:39-59. doi: 10.1385/1-59259-998-2:39.

The baculovirus/insect cell heterologous expression system provides an important tool for investigating the catalytic activity of individual drug-metabolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression...

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Janmohamed A, Thaunsukon P, Shephard EA, et al. Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods Mol Biol. 2006;320:39-59doi: 10.1385/1-59259-998-2:39.
Janmohamed, A., Thaunsukon, P., Shephard, E. A., & Phillips, I. R. (2006). Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods in molecular biology (Clifton, N.J.), 32039-59. https://doi.org/10.1385/1-59259-998-2:39
Janmohamed, Azara, et al. "Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System." Methods in molecular biology (Clifton, N.J.) vol. 320 (2006): 39-59. doi: https://doi.org/10.1385/1-59259-998-2:39
Janmohamed A, Thaunsukon P, Shephard EA, Phillips IR. Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System. Methods Mol Biol. 2006;320:39-59. doi: 10.1385/1-59259-998-2:39. PMID: 27699678.
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Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides.

Botanical studies

Huang SS, Deng JS, Chen HJ, Lin YH, Huang GJ.
PMID: 28510944
Bot Stud. 2014 Dec;55(1):64. doi: 10.1186/s40529-014-0064-4. Epub 2014 Aug 15.

BACKGROUND: Metallothionein (MT) characterized by their low molecular weight and high cysteine content.RESULTS: Two recombinant proteins of MT-I and MT-II overproduced in E. coli (M15) was purified by NiCONCLUSIONS: These findings mean that a cysteine residue is most important...

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Huang SS, Deng JS, Chen HJ, et al. Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides. Bot Stud. 2014;55(1):64doi: 10.1186/s40529-014-0064-4.
Huang, S. S., Deng, J. S., Chen, H. J., Lin, Y. H., & Huang, G. J. (2014). Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides. Botanical studies, 55(1), 64. https://doi.org/10.1186/s40529-014-0064-4
Huang, Shyh-Shyun, et al. "Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides." Botanical studies vol. 55,1 (2014): 64. doi: https://doi.org/10.1186/s40529-014-0064-4
Huang SS, Deng JS, Chen HJ, Lin YH, Huang GJ. Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57') storage roots and their synthesized peptides. Bot Stud. 2014 Dec;55(1):64. doi: 10.1186/s40529-014-0064-4. Epub 2014 Aug 15. PMID: 28510944; PMCID: PMC5430340.
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Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase.

Journal of applied microbiology

Rezaie F, Davami F, Mansouri K, Agha Amiri S, Fazel R, Mahdian R, Davoudi N, Enayati S, Azizi M, Khalaj V.
PMID: 28482126
J Appl Microbiol. 2017 Jul;123(1):134-144. doi: 10.1111/jam.13483. Epub 2017 Jun 09.

AIMS: The Escherichia coli expression system is highly effective in producing recombinant proteins. However, there are some limitations in this system, especially in obtaining correctly folded forms of some complex proteins such as Fab fragments. To improve the solubility...

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Rezaie F, Davami F, Mansouri K, et al. Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase. J Appl Microbiol. 2017;123(1):134-144doi: 10.1111/jam.13483.
Rezaie, F., Davami, F., Mansouri, K., Agha Amiri, S., Fazel, R., Mahdian, R., Davoudi, N., Enayati, S., Azizi, M., & Khalaj, V. (2017). Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase. Journal of applied microbiology, 123(1), 134-144. https://doi.org/10.1111/jam.13483
Rezaie, F, et al. "Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase." Journal of applied microbiology vol. 123,1 (2017): 134-144. doi: https://doi.org/10.1111/jam.13483
Rezaie F, Davami F, Mansouri K, Agha Amiri S, Fazel R, Mahdian R, Davoudi N, Enayati S, Azizi M, Khalaj V. Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase. J Appl Microbiol. 2017 Jul;123(1):134-144. doi: 10.1111/jam.13483. Epub 2017 Jun 09. PMID: 28482126.
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Diagnostic relevance of autoantibody detection against inhibitors of apoptosis proteins in colon cancer and colon adenoma.

Molecular and clinical oncology

Hosono Y, Goto M, Kobayashi D, Kuribayashi K, Tanaka M, Watanabe N.
PMID: 26137273
Mol Clin Oncol. 2015 May;3(3):595-600. doi: 10.3892/mco.2015.502. Epub 2015 Feb 06.

Autoantibodies against cancer-related antigens may be detected in the sera of patients with various types of cancer, although their clinical utility has not yet been established. In this study, we aimed to demonstrate the diagnostic relevance of autoantibody detection...

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Hosono Y, Goto M, Kobayashi D, et al. Diagnostic relevance of autoantibody detection against inhibitors of apoptosis proteins in colon cancer and colon adenoma. Mol Clin Oncol. 2015;3(3):595-600doi: 10.3892/mco.2015.502.
Hosono, Y., Goto, M., Kobayashi, D., Kuribayashi, K., Tanaka, M., & Watanabe, N. (2015). Diagnostic relevance of autoantibody detection against inhibitors of apoptosis proteins in colon cancer and colon adenoma. Molecular and clinical oncology, 3(3), 595-600. https://doi.org/10.3892/mco.2015.502
Hosono, Yukie, et al. "Diagnostic relevance of autoantibody detection against inhibitors of apoptosis proteins in colon cancer and colon adenoma." Molecular and clinical oncology vol. 3,3 (2015): 595-600. doi: https://doi.org/10.3892/mco.2015.502
Hosono Y, Goto M, Kobayashi D, Kuribayashi K, Tanaka M, Watanabe N. Diagnostic relevance of autoantibody detection against inhibitors of apoptosis proteins in colon cancer and colon adenoma. Mol Clin Oncol. 2015 May;3(3):595-600. doi: 10.3892/mco.2015.502. Epub 2015 Feb 06. PMID: 26137273; PMCID: PMC4471536.
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A novel baculovirus-derived promoter with high activity in the baculovirus expression system.

PeerJ

Martínez-Solís M, Gómez-Sebastián S, Escribano JM, Jakubowska AK, Herrero S.
PMID: 27375973
PeerJ. 2016 Jun 28;4:e2183. doi: 10.7717/peerj.2183. eCollection 2016.

The baculovirus expression vector system (BEVS) has been widely used to produce a large number of recombinant proteins, and is becoming one of the most powerful, robust, and cost-effective systems for the production of eukaryotic proteins. Nevertheless, as in...

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Martínez-Solís M, Gómez-Sebastián S, Escribano JM, et al. A novel baculovirus-derived promoter with high activity in the baculovirus expression system. PeerJ. 2016;4:e2183doi: 10.7717/peerj.2183.
Martínez-Solís, M., Gómez-Sebastián, S., Escribano, J. M., Jakubowska, A. K., & Herrero, S. (2016). A novel baculovirus-derived promoter with high activity in the baculovirus expression system. PeerJ, 4e2183. https://doi.org/10.7717/peerj.2183
Martínez-Solís, María, et al. "A novel baculovirus-derived promoter with high activity in the baculovirus expression system." PeerJ vol. 4 (2016): e2183. doi: https://doi.org/10.7717/peerj.2183
Martínez-Solís M, Gómez-Sebastián S, Escribano JM, Jakubowska AK, Herrero S. A novel baculovirus-derived promoter with high activity in the baculovirus expression system. PeerJ. 2016 Jun 28;4:e2183. doi: 10.7717/peerj.2183. eCollection 2016. PMID: 27375973; PMCID: PMC4928464.
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Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies.

AMB Express

Blanco-Toribio A, Álvarez-Cienfuegos A, Sainz-Pastor N, Merino N, Compte M, Sanz L, Blanco FJ, Álvarez-Vallina L.
PMID: 26239030
AMB Express. 2015 Dec;5(1):137. doi: 10.1186/s13568-015-0137-0. Epub 2015 Aug 04.

Recombinant antibodies are used with great success in many different diagnostic and therapeutic applications. A variety of protein expression systems are available, but nowadays almost all therapeutic antibodies are produced in mammalian cell lines due to their complex structure...

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Blanco-Toribio A, Álvarez-Cienfuegos A, Sainz-Pastor N, et al. Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies. AMB Express. 2015;5(1):137doi: 10.1186/s13568-015-0137-0.
Blanco-Toribio, A., Álvarez-Cienfuegos, A., Sainz-Pastor, N., Merino, N., Compte, M., Sanz, L., Blanco, F. J., & Álvarez-Vallina, L. (2015). Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies. AMB Express, 5(1), 137. https://doi.org/10.1186/s13568-015-0137-0
Blanco-Toribio, Ana, et al. "Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies." AMB Express vol. 5,1 (2015): 137. doi: https://doi.org/10.1186/s13568-015-0137-0
Blanco-Toribio A, Álvarez-Cienfuegos A, Sainz-Pastor N, Merino N, Compte M, Sanz L, Blanco FJ, Álvarez-Vallina L. Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies. AMB Express. 2015 Dec;5(1):137. doi: 10.1186/s13568-015-0137-0. Epub 2015 Aug 04. PMID: 26239030; PMCID: PMC4523561.
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Characterization of Nora Virus Structural Proteins via Western Blot Analysis.

Scientifica

Ericson BL, Carlson DJ, Carlson KA.
PMID: 27298753
Scientifica (Cairo). 2016;2016:9067848. doi: 10.1155/2016/9067848. Epub 2016 May 19.

Nora virus is a single stranded RNA picorna-like virus with four open reading frames (ORFs). The coding potentials of the ORFs are not fully characterized, but ORF3 and ORF4 are believed to encode the capsid proteins (VP3, VP4a, VP4b,...

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Ericson BL, Carlson DJ, Carlson KA. Characterization of Nora Virus Structural Proteins via Western Blot Analysis. Scientifica (Cairo). 2016;2016:9067848doi: 10.1155/2016/9067848.
Ericson, B. L., Carlson, D. J., & Carlson, K. A. (2016). Characterization of Nora Virus Structural Proteins via Western Blot Analysis. Scientifica, 20169067848. https://doi.org/10.1155/2016/9067848
Ericson, Brad L, et al. "Characterization of Nora Virus Structural Proteins via Western Blot Analysis." Scientifica vol. 2016 (2016): 9067848. doi: https://doi.org/10.1155/2016/9067848
Ericson BL, Carlson DJ, Carlson KA. Characterization of Nora Virus Structural Proteins via Western Blot Analysis. Scientifica (Cairo). 2016;2016:9067848. doi: 10.1155/2016/9067848. Epub 2016 May 19. PMID: 27298753; PMCID: PMC4889860.
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Quality of histone modification antibodies undermines chromatin biology research.

F1000Research

Kungulovski G, Jeltsch A.
PMID: 26834995
F1000Res. 2015 Oct 28;4:1160. doi: 10.12688/f1000research.7265.2. eCollection 2015.

Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by...

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Kungulovski G, Jeltsch A. Quality of histone modification antibodies undermines chromatin biology research. F1000Res. 2015;4:1160doi: 10.12688/f1000research.7265.2.
Kungulovski, G., & Jeltsch, A. (2015). Quality of histone modification antibodies undermines chromatin biology research. F1000Research, 41160. https://doi.org/10.12688/f1000research.7265.2
Kungulovski, Goran, and Jeltsch, Albert. "Quality of histone modification antibodies undermines chromatin biology research." F1000Research vol. 4 (2015): 1160. doi: https://doi.org/10.12688/f1000research.7265.2
Kungulovski G, Jeltsch A. Quality of histone modification antibodies undermines chromatin biology research. F1000Res. 2015 Oct 28;4:1160. doi: 10.12688/f1000research.7265.2. eCollection 2015. PMID: 26834995; PMCID: PMC4706057.
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Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development.

Frontiers in plant science

Spiegel H, Boes A, Voepel N, Beiss V, Edgue G, Rademacher T, Sack M, Schillberg S, Reimann A, Fischer R.
PMID: 26779197
Front Plant Sci. 2015 Dec 23;6:1169. doi: 10.3389/fpls.2015.01169. eCollection 2015.

Despite decades of intensive research efforts there is currently no vaccine that provides sustained sterile immunity against malaria. In this context, a large number of targets from the different stages of the Plasmodium falciparum life cycle have been evaluated...

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Spiegel H, Boes A, Voepel N, et al. Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Front Plant Sci. 2015;6:1169doi: 10.3389/fpls.2015.01169.
Spiegel, H., Boes, A., Voepel, N., Beiss, V., Edgue, G., Rademacher, T., Sack, M., Schillberg, S., Reimann, A., & Fischer, R. (2015). Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Frontiers in plant science, 61169. https://doi.org/10.3389/fpls.2015.01169
Spiegel, Holger, et al. "Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development." Frontiers in plant science vol. 6 (2015): 1169. doi: https://doi.org/10.3389/fpls.2015.01169
Spiegel H, Boes A, Voepel N, Beiss V, Edgue G, Rademacher T, Sack M, Schillberg S, Reimann A, Fischer R. Application of a Scalable Plant Transient Gene Expression Platform for Malaria Vaccine Development. Front Plant Sci. 2015 Dec 23;6:1169. doi: 10.3389/fpls.2015.01169. eCollection 2015. PMID: 26779197; PMCID: PMC4688378.
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Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection.

International journal of mycobacteriology

Tebianian M, Mosavari N, Taghizadeh M, Ebrahimi SM.
PMID: 28043591
Int J Mycobacteriol. 2016 Dec;5:S254. doi: 10.1016/j.ijmyco.2016.11.011. Epub 2016 Nov 24.

INTRODUCTION: Since an accurate test for detection of Mycobacterium tuberculosis early infection is urgently needed, this study was designed for development of an efficient screening test in diagnosis of tuberculosis infection.MATERIALS AND METHODS: In the present study, two recombinant...

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Tebianian M, Mosavari N, Taghizadeh M, et al. Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. Int J Mycobacteriol. 2016;5:S254doi: 10.1016/j.ijmyco.2016.11.011.
Tebianian, M., Mosavari, N., Taghizadeh, M., & Ebrahimi, S. M. (2016). Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. International journal of mycobacteriology, 5S254. https://doi.org/10.1016/j.ijmyco.2016.11.011
Tebianian, Majid, et al. "Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection." International journal of mycobacteriology vol. 5 (2016): S254. doi: https://doi.org/10.1016/j.ijmyco.2016.11.011
Tebianian M, Mosavari N, Taghizadeh M, Ebrahimi SM. Evaluation of specific antibodies against Mycobacterium tuberculosis recombinant antigens for detection of recent infection. Int J Mycobacteriol. 2016 Dec;5:S254. doi: 10.1016/j.ijmyco.2016.11.011. Epub 2016 Nov 24. PMID: 28043591.
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Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during .

Biochemical engineering journal

Barretto SS, Michoux F, Hellgardt K, Nixon PJ.
PMID: 28111521
Biochem Eng J. 2017 Jan 15;117:73-81. doi: 10.1016/j.bej.2016.10.007.

Transplastomic plants are capable of high-yield production of recombinant biopharmaceutical proteins. Plant tissue culture combines advantages of agricultural cultivation with the bioprocess consistency associated with suspension culture. Overexpression of recombinant proteins through regeneration of transplastomic

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Barretto SS, Michoux F, Hellgardt K, et al. Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochem Eng J. 2017;117:73-81doi: 10.1016/j.bej.2016.10.007.
Barretto, S. S., Michoux, F., Hellgardt, K., & Nixon, P. J. (2017). Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochemical engineering journal, 11773-81. https://doi.org/10.1016/j.bej.2016.10.007
Barretto, Sherwin S, et al. "Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during ." Biochemical engineering journal vol. 117 (2017): 73-81. doi: https://doi.org/10.1016/j.bej.2016.10.007
Barretto SS, Michoux F, Hellgardt K, Nixon PJ. Pneumatic hydrodynamics influence transplastomic protein yields and biological responses during . Biochem Eng J. 2017 Jan 15;117:73-81. doi: 10.1016/j.bej.2016.10.007. PMID: 28111521; PMCID: PMC5221668.
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Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis.

Scientific reports

Schoonen L, van Esterik KS, Zhang C, Ulijn RV, Nolte RJM, Hest JCMV.
PMID: 29116178
Sci Rep. 2017 Nov 07;7(1):14772. doi: 10.1038/s41598-017-15310-y.

Hexahistidines are very common tags used in the affinity chromatography purification of recombinant proteins. Although these tags are solely applied for their metal-binding properties, we found that they are also able to perform ester hydrolysis when attached to a...

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Schoonen L, van Esterik KS, Zhang C, et al. Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Sci Rep. 2017;7(1):14772doi: 10.1038/s41598-017-15310-y.
Schoonen, L., van Esterik, K. S., Zhang, C., Ulijn, R. V., Nolte, R. J. M., & Hest, J. C. M. V. (2017). Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Scientific reports, 7(1), 14772. https://doi.org/10.1038/s41598-017-15310-y
Schoonen, Lise, et al. "Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis." Scientific reports vol. 7,1 (2017): 14772. doi: https://doi.org/10.1038/s41598-017-15310-y
Schoonen L, van Esterik KS, Zhang C, Ulijn RV, Nolte RJM, Hest JCMV. Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Sci Rep. 2017 Nov 07;7(1):14772. doi: 10.1038/s41598-017-15310-y. PMID: 29116178; PMCID: PMC5676709.
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Showing 1 to 12 of 1326 entries