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New Insights into the Classification and Integration Specificity of Streptococcus Integrative Conjugative Elements through Extensive Genome Exploration.

Frontiers in microbiology

Ambroset C, Coluzzi C, Guédon G, Devignes MD, Loux V, Lacroix T, Payot S, Leblond-Bourget N.
PMID: 26779141
Front Microbiol. 2016 Jan 06;6:1483. doi: 10.3389/fmicb.2015.01483. eCollection 2015.

Recent genome analyses suggest that integrative and conjugative elements (ICEs) are widespread in bacterial genomes and therefore play an essential role in horizontal transfer. However, only a few of these elements are precisely characterized and correctly delineated within sequenced...

Genome Sequences of Gordonia Phages Bowser and Schwabeltier.

Genome announcements

Montgomery MT, Pope WH, Arnold ZM, Basina A, Iyer AM, Stoner TH, Kasturiarachi NS, Pressimone CA, Schiebel JG, Furbee EC, Grubb SR, Warner MH, Garlena RA, Russell DA, Jacobs-Sera D, Hatfull GF.
PMID: 27516498
Genome Announc. 2016 Aug 11;4(4). doi: 10.1128/genomeA.00596-16.

Gordonia phages Bowser and Schwabeltier are newly isolated phages infecting Gordonia terrae 3612. Bowser and Schwabeltier have similar siphoviral morphologies and their genomes are related to each other, but not to other phages. Their lysis cassettes are atypically situated...

Retroviral DNA Transposition: Themes and Variations.

Microbiology spectrum

Skalka AM.
PMID: 25844274
Microbiol Spectr. 2014 Dec;2(5):MDNA300052014. doi: 10.1128/microbiolspec.MDNA3-0005-2014.

Retroviruses and LTR retrotransposons are transposable elements that encapsidate the RNAs that are intermediates in the transposition of DNA copies of their genomes (proviruses), from one cell (or one locus) to another. Mechanistic similarities in DNA transposase enzymes and...

Genomic comparison of invasive and rare non-invasive strains reveals Porphyromonas gingivalis genetic polymorphisms.

Journal of oral microbiology

Dolgilevich S, Rafferty B, Luchinskaya D, Kozarov E.
PMID: 21541093
J Oral Microbiol. 2011 Mar 09;3. doi: 10.3402/jom.v3i0.5764.

BACKGROUND: Porphyromonas gingivalis strains are shown to invade human cells in vitro with different invasion efficiencies, varying by up to three orders of magnitude.OBJECTIVE: We tested the hypothesis that invasion-associated interstrain genomic polymorphisms are present in P. gingivalis and...

Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons.

Mobile DNA

Cambray G, Sanchez-Alberola N, Campoy S, Guerin É, Da Re S, González-Zorn B, Ploy MC, Barbé J, Mazel D, Erill I.
PMID: 21529368
Mob DNA. 2011 Apr 30;2(1):6. doi: 10.1186/1759-8753-2-6.

BACKGROUND: Integrons are found in hundreds of environmental bacterial species, but are mainly known as the agents responsible for the capture and spread of antibiotic-resistance determinants between Gram-negative pathogens. The SOS response is a regulatory network under control of...

Phylogenetic structure and evolution of regulatory genes and integrases of P2-like phages.

Bacteriophage

Nilsson H, Cardoso-Palacios C, Haggård-Ljungquist E, Nilsson AS.
PMID: 23050214
Bacteriophage. 2011 Jul 01;1(4):207-218. doi: 10.4161/bact.1.4.18470.

The phylogenetic relationships and structural similarities of the proteins encoded within the regulatory region (containing the integrase gene and the lytic-lysogenic transcriptional switch genes) of P2-like phages were analyzed, and compared with the phylogenetic relationship of P2-like phages inferred...

The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-.

PeerJ

Herisse M, Porter JL, Guerillot R, Tomita T, Goncalves Da Silva A, Seemann T, Howden BP, Stinear TP, Pidot SJ.
PMID: 29740520
PeerJ. 2018 May 04;6:e4784. doi: 10.7717/peerj.4784. eCollection 2018.

Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited. Site specific integrases catalyze recombination between phage and bacterial...

A novel approach for T7 bacteriophage genome integration of exogenous DNA.

Journal of biological engineering

Liu Y, Huang H, Wang H, Zhang Y.
PMID: 31988659
J Biol Eng. 2020 Jan 16;14:2. doi: 10.1186/s13036-019-0224-x. eCollection 2020.

BACKGROUND: The comparatively small genome, well elucidated functional genomics and rapid life cycle confer T7 bacteriophage with great advantages for bio-application. Genetic manipulation of T7 genome plays a key role in T7 related applications. As one of the important...

Archaeal tyrosine recombinases.

FEMS microbiology reviews

Badel C, Da Cunha V, Oberto J.
PMID: 33524101
FEMS Microbiol Rev. 2021 Aug 17;45(4). doi: 10.1093/femsre/fuab004.

The integration of mobile genetic elements into their host chromosome influences the immediate fate of cellular organisms and gradually shapes their evolution. Site-specific recombinases catalyzing this integration have been extensively characterized both in bacteria and eukarya. More recently, a...

Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments.

Environmental pollution (Barking, Essex : 1987)

Di Cesare A, Sabatino R, Yang Y, Brambilla D, Li P, Fontaneto D, Eckert EM, Corno G.
PMID: 34974089
Environ Pollut. 2021 Dec 30;297:118774. doi: 10.1016/j.envpol.2021.118774. Epub 2021 Dec 30.

Wastewater treatment plants (WWTPs) are among the main hotspots of antibiotic resistance genes (ARGs) in the environment. Previously, we demonstrated that, by increasing anthropogenic pollution, the antibiotic resistome persisted in the microbial community of rivers and lakes, independently by...

The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in .

ACS synthetic biology

Hao N, Chen Q, Dodd IB, Shearwin KE.
PMID: 34190535
ACS Synth Biol. 2021 Jul 16;10(7):1633-1639. doi: 10.1021/acssynbio.1c00215. Epub 2021 Jun 30.

We describe a new set of tools for inserting DNA into the bacterial chromosome. The system uses site-specific recombination reactions carried out by bacteriophage integrases to integrate plasmids at up to eight phage attachment sites in

Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases.

Biomolecules

Rabe AJ, Tan YY, Larue RC, Yoder KE.
PMID: 34944553
Biomolecules. 2021 Dec 20;11(12). doi: 10.3390/biom11121910.

Integrases of different retroviruses assemble as functional complexes with varying multimers of the protein. Retroviral integrases require a divalent metal cation to perform one-step transesterification catalysis. Tetrameric prototype foamy virus (PFV) intasomes assembled from purified integrase and viral DNA...

Showing 1 to 12 of 60 entries