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Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification.

Chemical science

Wang H, Wang H, Duan X, Sun Y, Wang X, Li Z.
PMID: 28580102
Chem Sci. 2017 May 01;8(5):3635-3640. doi: 10.1039/c7sc00094d. Epub 2017 Mar 01.

Alternative messenger RNA (mRNA) splicing is a basic mechanism of gene regulation. In general, reverse transcription and polymerase based primer extension limit the sensitivity and selectivity of the current detection of mRNA splice variants, respectively. Here, we show that,...

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Wang H, Wang H, Duan X, et al. Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification. Chem Sci. 2017;8(5):3635-3640doi: 10.1039/c7sc00094d.
Wang, H., Wang, H., Duan, X., Sun, Y., Wang, X., & Li, Z. (2017). Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification. Chemical science, 8(5), 3635-3640. https://doi.org/10.1039/c7sc00094d
Wang, Honghong, et al. "Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification." Chemical science vol. 8,5 (2017): 3635-3640. doi: https://doi.org/10.1039/c7sc00094d
Wang H, Wang H, Duan X, Sun Y, Wang X, Li Z. Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification. Chem Sci. 2017 May 01;8(5):3635-3640. doi: 10.1039/c7sc00094d. Epub 2017 Mar 01. PMID: 28580102; PMCID: PMC5437374.
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Utilization of Human Papillomavirus DNA Detection for Cervical Cancer Screening in Women Presenting With Abnormal Cytology in Lokoja, Nigeria.

Jundishapur journal of microbiology

Kolawole O, Ogah J, Alabi O, Suleiman M, Amuda O, Kolawole F.
PMID: 26568803
Jundishapur J Microbiol. 2015 Oct 20;8(10):e22620. doi: 10.5812/jjm.22620. eCollection 2015 Oct.

BACKGROUND: Cervical cancer is regarded as the second highest cause of cancer deaths in Nigeria, with an overall prevalence similar to most developing countries. Screening for cervical cancer is primarily performed using papanicolaou (PAP) staining procedure, in Nigeria.OBJECTIVES: This...

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Kolawole O, Ogah J, Alabi O, et al. Utilization of Human Papillomavirus DNA Detection for Cervical Cancer Screening in Women Presenting With Abnormal Cytology in Lokoja, Nigeria. Jundishapur J Microbiol. 2015;8(10):e22620doi: 10.5812/jjm.22620.
Kolawole, O., Ogah, J., Alabi, O., Suleiman, M., Amuda, O., & Kolawole, F. (2015). Utilization of Human Papillomavirus DNA Detection for Cervical Cancer Screening in Women Presenting With Abnormal Cytology in Lokoja, Nigeria. Jundishapur journal of microbiology, 8(10), e22620. https://doi.org/10.5812/jjm.22620
Kolawole, Olatunji, et al. "Utilization of Human Papillomavirus DNA Detection for Cervical Cancer Screening in Women Presenting With Abnormal Cytology in Lokoja, Nigeria." Jundishapur journal of microbiology vol. 8,10 (2015): e22620. doi: https://doi.org/10.5812/jjm.22620
Kolawole O, Ogah J, Alabi O, Suleiman M, Amuda O, Kolawole F. Utilization of Human Papillomavirus DNA Detection for Cervical Cancer Screening in Women Presenting With Abnormal Cytology in Lokoja, Nigeria. Jundishapur J Microbiol. 2015 Oct 20;8(10):e22620. doi: 10.5812/jjm.22620. eCollection 2015 Oct. PMID: 26568803; PMCID: PMC4640102.
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Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Beckmann JS, Soller M.
PMID: 24258478
Theor Appl Genet. 1983 Nov;67(1):35-43. doi: 10.1007/BF00303919.

Recently a new class of genetic polymorphism, restriction fragment length polymorphisms (RFLPs), has been uncovered by the use of restriction endonucleases which cleave DNA molecules at specific sites and cloned DNA probes which detect specific homologous DNA fragments. RFLPs...

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Beckmann JS, Soller M. Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet. 1983;67(1):35-43doi: 10.1007/BF00303919.
Beckmann, J. S., & Soller, M. (1983). Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 67(1), 35-43. https://doi.org/10.1007/BF00303919
Beckmann, J S, and Soller, M. "Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 67,1 (1983): 35-43. doi: https://doi.org/10.1007/BF00303919
Beckmann JS, Soller M. Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet. 1983 Nov;67(1):35-43. doi: 10.1007/BF00303919. PMID: 24258478.
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Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Xu YS, Jones MG, Karp A, Pehu E.
PMID: 24196153
Theor Appl Genet. 1993 Feb;85(8):1017-22. doi: 10.1007/BF00215042.

Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or...

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Xu YS, Jones MG, Karp A, et al. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. Theor Appl Genet. 1993;85(8):1017-22doi: 10.1007/BF00215042.
Xu, Y. S., Jones, M. G., Karp, A., & Pehu, E. (1993). Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 85(8), 1017-22. https://doi.org/10.1007/BF00215042
Xu, Y S, et al. "Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 85,8 (1993): 1017-22. doi: https://doi.org/10.1007/BF00215042
Xu YS, Jones MG, Karp A, Pehu E. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. Theor Appl Genet. 1993 Feb;85(8):1017-22. doi: 10.1007/BF00215042. PMID: 24196153.
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The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Sanz-Alferez S, Richter TE, Hulbert SH, Bennetzen JL.
PMID: 24169663
Theor Appl Genet. 1995 Jul;91(1):25-32. doi: 10.1007/BF00220854.

The Rp3 locus of maize conditions race-specific resistance to a fungal rust pathogen, Puccinia sorghi. Both morphological and DNA markers were employed to characterize alleles of Rp3 and to accurately position Rp3 on the maize genetic map. DNA marker...

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Sanz-Alferez S, Richter TE, Hulbert SH, et al. The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles. Theor Appl Genet. 1995;91(1):25-32doi: 10.1007/BF00220854.
Sanz-Alferez, S., Richter, T. E., Hulbert, S. H., & Bennetzen, J. L. (1995). The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 91(1), 25-32. https://doi.org/10.1007/BF00220854
Sanz-Alferez, S, et al. "The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 91,1 (1995): 25-32. doi: https://doi.org/10.1007/BF00220854
Sanz-Alferez S, Richter TE, Hulbert SH, Bennetzen JL. The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles. Theor Appl Genet. 1995 Jul;91(1):25-32. doi: 10.1007/BF00220854. PMID: 24169663.
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Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification.

Pathogens (Basel, Switzerland)

Akama T, Kawashima A, Tanigawa K, Hayashi M, Ishido Y, Luo Y, Hata A, Fujitani N, Ishii N, Suzuki K.
PMID: 25437334
Pathogens. 2013 Oct 30;2(4):591-605. doi: 10.3390/pathogens2040591.

The microflora in environmental water consists of a high density and diversity of bacterial species that form the foundation of the water ecosystem. Because the majority of these species cannot be cultured in vitro, a different approach is needed...

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Akama T, Kawashima A, Tanigawa K, et al. Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification. Pathogens. 2013;2(4):591-605doi: 10.3390/pathogens2040591.
Akama, T., Kawashima, A., Tanigawa, K., Hayashi, M., Ishido, Y., Luo, Y., Hata, A., Fujitani, N., Ishii, N., & Suzuki, K. (2013). Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification. Pathogens (Basel, Switzerland), 2(4), 591-605. https://doi.org/10.3390/pathogens2040591
Akama, Takeshi, et al. "Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification." Pathogens (Basel, Switzerland) vol. 2,4 (2013): 591-605. doi: https://doi.org/10.3390/pathogens2040591
Akama T, Kawashima A, Tanigawa K, Hayashi M, Ishido Y, Luo Y, Hata A, Fujitani N, Ishii N, Suzuki K. Comprehensive analysis of prokaryotes in environmental water using DNA microarray analysis and whole genome amplification. Pathogens. 2013 Oct 30;2(4):591-605. doi: 10.3390/pathogens2040591. PMID: 25437334; PMCID: PMC4235703.
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Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells.

Planta

Saxena PK, Mii M, Crosby WL, Fowke LC, King J.
PMID: 24233731
Planta. 1986 May;168(1):29-35. doi: 10.1007/BF00407005.

The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca(2+) at pH 6.8. The frequency of nuclear...

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Saxena PK, Mii M, Crosby WL, et al. Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta. 1986;168(1):29-35doi: 10.1007/BF00407005.
Saxena, P. K., Mii, M., Crosby, W. L., Fowke, L. C., & King, J. (1986). Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta, 168(1), 29-35. https://doi.org/10.1007/BF00407005
Saxena, P K, et al. "Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells." Planta vol. 168,1 (1986): 29-35. doi: https://doi.org/10.1007/BF00407005
Saxena PK, Mii M, Crosby WL, Fowke LC, King J. Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta. 1986 May;168(1):29-35. doi: 10.1007/BF00407005. PMID: 24233731.
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Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Derks FH, Hakkert JC, Verbeek WH, Colijn-Hooymans CM.
PMID: 24201496
Theor Appl Genet. 1992 Sep;84(7):930-40. doi: 10.1007/BF00227406.

A series of fusion experiments were performed between protoplasts of a cytoplasmic albino mutant of tomato, Lycopersicon esculentum (ALRC), and gamma-irradiated protoplasts of L. hirsutum and the Solanum species S. commersonii, S. etuberosum and S. nigrum. These species were...

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Derks FH, Hakkert JC, Verbeek WH, et al. Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction. Theor Appl Genet. 1992;84(7):930-40doi: 10.1007/BF00227406.
Derks, F. H., Hakkert, J. C., Verbeek, W. H., & Colijn-Hooymans, C. M. (1992). Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 84(7), 930-40. https://doi.org/10.1007/BF00227406
Derks, F H, et al. "Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 84,7 (1992): 930-40. doi: https://doi.org/10.1007/BF00227406
Derks FH, Hakkert JC, Verbeek WH, Colijn-Hooymans CM. Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction. Theor Appl Genet. 1992 Sep;84(7):930-40. doi: 10.1007/BF00227406. PMID: 24201496.
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Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study.

Journal of pathology and translational medicine

Choi SE, Hong SW, Yoon SO.
PMID: 26018515
J Pathol Transl Med. 2015 May;49(3):236-42. doi: 10.4132/jptm.2015.03.16. Epub 2015 May 15.

BACKGROUND: The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material...

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Choi SE, Hong SW, Yoon SO. Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study. J Pathol Transl Med. 2015;49(3):236-42doi: 10.4132/jptm.2015.03.16.
Choi, S. E., Hong, S. W., & Yoon, S. O. (2015). Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study. Journal of pathology and translational medicine, 49(3), 236-42. https://doi.org/10.4132/jptm.2015.03.16
Choi, Sung-Eun, et al. "Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study." Journal of pathology and translational medicine vol. 49,3 (2015): 236-42. doi: https://doi.org/10.4132/jptm.2015.03.16
Choi SE, Hong SW, Yoon SO. Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study. J Pathol Transl Med. 2015 May;49(3):236-42. doi: 10.4132/jptm.2015.03.16. Epub 2015 May 15. PMID: 26018515; PMCID: PMC4440935.
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Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains.

Planta

Cornford CA, Black M, Chapman JM, Baulcombe DC.
PMID: 24232656
Planta. 1986 Nov;169(3):420-8. doi: 10.1007/BF00392140.

Aleurone tissue from freshly harvested immature wheat grains (Triticum aestivum L. cv. Sappo) which is normally unresponsive to gibberellic acid can be made responsive by subjecting the tissue to a pre-incubation treatment in a simple buffered medium prior to...

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Cornford CA, Black M, Chapman JM, et al. Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains. Planta. 1986;169(3):420-8doi: 10.1007/BF00392140.
Cornford, C. A., Black, M., Chapman, J. M., & Baulcombe, D. C. (1986). Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains. Planta, 169(3), 420-8. https://doi.org/10.1007/BF00392140
Cornford, C A, et al. "Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains." Planta vol. 169,3 (1986): 420-8. doi: https://doi.org/10.1007/BF00392140
Cornford CA, Black M, Chapman JM, Baulcombe DC. Expression of α-amylase and other gibberellin-regulated genes in aleurone tissue of developing wheat grains. Planta. 1986 Nov;169(3):420-8. doi: 10.1007/BF00392140. PMID: 24232656.
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X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Imamura J, Saul MW, Potrykus I.
PMID: 24241798
Theor Appl Genet. 1987 Aug;74(4):445-50. doi: 10.1007/BF00289819.

Complementation of two metabolic deficiences - nitrate reductase and tryptophan synthase - was used to select for somatic fusion hybrids between tobacco (Nicotiana tabacum) and henbane (Hyoscyamus muticus) with prior X-irradiation of one partner. Using species specific, radioactively labelled...

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Imamura J, Saul MW, Potrykus I. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor Appl Genet. 1987;74(4):445-50doi: 10.1007/BF00289819.
Imamura, J., Saul, M. W., & Potrykus, I. (1987). X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 74(4), 445-50. https://doi.org/10.1007/BF00289819
Imamura, J, et al. "X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 74,4 (1987): 445-50. doi: https://doi.org/10.1007/BF00289819
Imamura J, Saul MW, Potrykus I. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor Appl Genet. 1987 Aug;74(4):445-50. doi: 10.1007/BF00289819. PMID: 24241798.
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Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro.

Plant molecular biology

Pfeiffer P, Laquel P, Hohn T.
PMID: 24310510
Plant Mol Biol. 1984 Sep;3(5):261-70. doi: 10.1007/BF00017779.

The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in...

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Pfeiffer P, Laquel P, Hohn T. Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro. Plant Mol Biol. 1984;3(5):261-70doi: 10.1007/BF00017779.
Pfeiffer, P., Laquel, P., & Hohn, T. (1984). Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro. Plant molecular biology, 3(5), 261-70. https://doi.org/10.1007/BF00017779
Pfeiffer, P, et al. "Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro." Plant molecular biology vol. 3,5 (1984): 261-70. doi: https://doi.org/10.1007/BF00017779
Pfeiffer P, Laquel P, Hohn T. Cauliflower Mosaic Virus replication complexes: characterization of the associated enzymes and of the polarity of the DNA synthesized in vitro. Plant Mol Biol. 1984 Sep;3(5):261-70. doi: 10.1007/BF00017779. PMID: 24310510.
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Showing 1 to 12 of 412 entries