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Showing 1 to 12 of 177 entries
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Nodulins and nodulin genes of Glycine max.

Plant molecular biology

Verma DP, Fortin MG, Stanley J, Mauro VP, Purohit S, Morrison N.
PMID: 24302157
Plant Mol Biol. 1986 Jan;7(1):51-61. doi: 10.1007/BF00020131.

Nodulins are organ-specific plant proteins induced during symbiotic nitrogen fixation. Nodulins play both metabolic and structural roles within infected and uninfected nodule cells. In soybean, several nodulin genes, coding for abundant nodulins, have been identified and isolated. Structural analysis...

Type III chaperones & Co in bacterial plant pathogens: a set of specialized bodyguards mediating effector delivery.

Frontiers in plant science

Lohou D, Lonjon F, Genin S, Vailleau F.
PMID: 24319448
Front Plant Sci. 2013 Nov 22;4:435. doi: 10.3389/fpls.2013.00435.

Gram-negative plant pathogenic bacteria possess a type III secretion system (T3SS) to inject bacterial proteins, called type III effectors (T3Es), into host cells through a specialized syringe structure. T3Es are virulence factors that can suppress plant immunity but they...

Cloning and Expression of Annexin V cDNA in E.coli.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Sun JX, Shen XC, Wu XF.
PMID: 12136208
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(2):163-166.

The cDNA encoding the mature annexin V was isolated by using RT-PCR method from total RNAs of fresh human placenta. The result of sequencing indicated that the sequence of isolated annexin V cDNA was the same as the reported...

Recombinant Human DFF45 Inhibits Apoptosis-specific Endonuclease in a Cell-free System of Xenopus Egg Extracts.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Yang W, Lu ZG, Zhang L, Zhai ZH.
PMID: 12053194
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2001;33(1):82-86.

The human DNA fragmentation factor (DFF) is a heterodimer of 40 kD and 45 kD subunits. The 40 kD subunit (DFF40) has an intrinsic DNase activity responsible for the genomic DNA degradation into nucleosomal fragments during apoptosis. As an...

His(6) Fusion Expression of Myristoyl-CoA: Protein N-myristoyltransferase in E. coli and its Purification.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Xu ZP, Duan ZJ, Chen CZ, Yang XY, Li BL.
PMID: 12215804
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(4):383-388.

Saccharomyces cerevisiae Myristoyl-CoA: protein N-myristoyltransferase (NMT) gene was cloned into a His(6)-fusion expression vector pMFHT. After transforming into E. coli BL21 (DE3), His(6)-NMT was induced to express at 37 degrees by IPTG. SDS-PAGE analysis showed an induced expression product...

Development of Contamination-Free Restriction Fragment Length Polymorphism Probes for the Obligate Biotroph Peronospora tabacina, an Oomycete Causing Blue Mold of Tobacco.

Phytopathology

Sukno SA, Taylor AM, Farman M.
PMID: 18944249
Phytopathology. 2002 Nov;92(11):1227-35. doi: 10.1094/PHYTO.2002.92.11.1227.

ABSTRACT Peronospora tabacina is an obligately parasitic oomycete that causes blue mold, a devastating disease of tobacco. Genetic studies of this pathogen have been hampered by the lack of molecular markers. We generated a set of molecular markers for...

Expression of bacterial genes in transgenic tobacco: methods, applications and future prospects.

Electronic journal of biotechnology : EJB

Jube S, Borthakur D.
PMID: 19750137
Electron J Biotechnol. 2007 Jul 15;10(3):452-467. doi: 10.2225/vol10-issue3-fulltext-4.

Tobacco is the most commonly used plant for expression of transgenes from a variety of organisms, because it is easily grown and transformed, it provides abundant amounts of fresh tissue and has a well-established cell culture system. Many bacterial...

SEREX, Proteomex, AMIDA, and beyond: Serological screening technologies for target identification.

Proteomics. Clinical applications

Rauch J, Gires O.
PMID: 21136839
Proteomics Clin Appl. 2008 Mar;2(3):355-71. doi: 10.1002/prca.200780064. Epub 2008 Feb 07.

Despite the great body of knowledge about the aetiology, pathogenesis, risk factors, and associated molecular processes, cancer remains a prime health concern. Over the past decades scientific and medical research focused on the identification of biomarkers and target molecules...

The effect of osmotic shock on release of bacterial proteins and on active transport.

The Journal of general physiology

Heppel LA.
PMID: 19873660
J Gen Physiol. 1969 Jul 01;54(1):95-113. doi: 10.1085/jgp.54.1.95.

Osmotic shock is a procedure in which Gram-negative bacteria are treated as follows. First they are suspended in 0.5 M sucrose containing ethylenediaminetetraacetate. After removal of the sucrose by centrifugation, the pellet of cells is rapidly dispersed in cold,...

From host recognition to T-DNA integration: the function of bacterial and plant genes in the Agrobacterium-plant cell interaction.

Molecular plant pathology

Tzfira T, Citovsky V.
PMID: 20572967
Mol Plant Pathol. 2000 Jul 01;1(4):201-12. doi: 10.1046/j.1364-3703.2000.00026.x.

UNLABELLED: Abstract Agrobacterium tumefaciens and its related species, A. rhizogenes and A. vitis, are the only known bacterial pathogens which 'genetically invade' host plants and stably integrate part of their genetic material into the host cell genome. Thus, A....

Control of host cell phosphorylation by legionella pneumophila.

Frontiers in microbiology

Haenssler E, Isberg RR.
PMID: 21747787
Front Microbiol. 2011 Apr 04;2:64. doi: 10.3389/fmicb.2011.00064. eCollection 2011.

Phosphorylation is one of the most frequent modifications in intracellular signaling and is implicated in many processes ranging from transcriptional control to signal transduction in innate immunity. Many pathogens modulate host cell phosphorylation pathways to promote growth and establish...

THE RETENTION OF FOREIGN PROTEIN BY THE KIDNEY : A STUDY IN ANAPHYLAXIS.

The Journal of experimental medicine

Pearce RM.
PMID: 19867579
J Exp Med. 1912 Sep 01;16(3):349-62. doi: 10.1084/jem.16.3.349.

Extracts of the kidneys of normal rabbits prepared one, two, three, and four days after the intravenous injection of egg-albumin and horse serum have the power to sensitize guinea pigs to a second injection of these proteins. The sensitization...

Showing 1 to 12 of 177 entries